Dietary and bioactive properties of the berries and leaves from the underutilized Hippophae salicifolia D. Don grown in Northeast India

Abstract

The physico-chemical, polyphenols, antioxidant and antibacterial properties of berries and mixture of male and female leaves of Hippophae salicifolia were investigated. The mineral, vitamin C, sugar, total protein, and total tannin contents of the berries and the leaves were evaluated. Further, the extracts of berries and mixture of leaves samples obtained by successive solvent extraction were investigated for their polyphenols, antioxidant and antibacterial properties. Total phenolic content was highest in leaves-methanol extract (157.97 +/- 2.09 mg GAE/g) followed by berries-aqueous extract (48.45 +/- 1.94 mg GAE/g), while total flavonoid was predominant in leaves-acetone extract (75.64 +/- 3.21 mg QE/g) and berries-methanol extract (28.93 +/- 2.08 mg QE/g). Gallic acid, caffeic acid, and rutin were the major polyphenols confirmed by HPLC analysis. Berries-aqueous and leaves-methanol extracts showed excellent global antioxidant score. Best antibacterial activity was observed by methanol extracts against eight different strains. Overall, the leaves and berries of Hippophae salicifolia collected from Northeast India exhibited good antioxidant and antibacterial activity and can be utilized by food and pharmaceutical sectors.