Vascular Endothelial Growth Factor Receptor 1 Targeting Fusion Polypeptides with Stimuli-Responsiveness for Anti-angiogenesis
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, Min Jeong | - |
dc.contributor.author | Roh, Kug-Hwan | - |
dc.contributor.author | Lee, Jae Sang | - |
dc.contributor.author | Lee, Jae Hee | - |
dc.contributor.author | Park, SaeGwang | - |
dc.contributor.author | Lim, Dong Woo | - |
dc.date.accessioned | 2023-07-24T09:44:12Z | - |
dc.date.available | 2023-07-24T09:44:12Z | - |
dc.date.created | 2023-07-17 | - |
dc.date.issued | 2023-06 | - |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/187446 | - |
dc.description.abstract | Genetically engineered fusion polypeptideshave beeninvestigatedto introduce unique bio-functionality and improve some therapeuticactivity for anti-angiogenesis. We report herein that stimuli-responsive,vascular endothelial growth factor receptor 1 (VEGFR1) targeting fusionpolypeptides composed of a VEGFR1 (fms-like tyrosine kinase-1 (Flt1))antagonist, an anti-Flt1 peptide, and a thermally responsive elastin-basedpolypeptide (EBP) were rationally designed at the genetic level, biosynthesized,and purified by inverse transition cycling to develop potential anti-angiogenicfusion polypeptides to treat neovascular diseases. A series of hydrophilicEBPs with different block lengths were fused with an anti-Flt1 peptide,forming anti-Flt1-EBPs, and the effect of EBP block length on theirphysicochemical properties was examined. While the anti-Flt1 peptidedecreased phase-transition temperatures of anti-Flt1-EBPs, comparedwith EBP blocks, anti-Flt1-EBPs were soluble under physiological conditions.The anti-Flt1-EBPs dose dependently inhibited the binding of VEGFR1against vascular endothelial growth factor (VEGF) as well as tube-likenetwork formation of human umbilical vein endothelial cells underVEGF-triggered angiogenesis in vitro because of the specific bindingbetween anti-Flt1-EBPs and VEGFR1. Furthermore, the anti-Flt1-EBPssuppressed laser-induced choroidal neovascularization in a wet age-relatedmacular degeneration mouse model in vivo. Our results indicate thatanti-Flt1-EBPs as VEGFR1-targeting fusion polypeptides have greatpotential for efficacious anti-angiogenesis to treat retinal-, corneal-,and choroidal neovascularization. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | American Chemical Society | - |
dc.title | Vascular Endothelial Growth Factor Receptor 1 Targeting Fusion Polypeptides with Stimuli-Responsiveness for Anti-angiogenesis | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lim, Dong Woo | - |
dc.identifier.doi | 10.1021/acsami.3c03989 | - |
dc.identifier.wosid | 001016557000001 | - |
dc.identifier.bibliographicCitation | ACS Applied Materials & Interfaces, pp.1 - 14 | - |
dc.relation.isPartOf | ACS Applied Materials & Interfaces | - |
dc.citation.title | ACS Applied Materials & Interfaces | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 14 | - |
dc.type.rims | ART | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | ELASTIN-LIKE POLYPEPTIDE | - |
dc.subject.keywordPlus | PEPTIDE-HYALURONATE CONJUGATE | - |
dc.subject.keywordPlus | ANTI-FLT1 PEPTIDE | - |
dc.subject.keywordPlus | RETINAL NEOVASCULARIZATION | - |
dc.subject.keywordPlus | RECOMBINANT PROTEIN | - |
dc.subject.keywordPlus | BEVACIZUMAB AVASTIN | - |
dc.subject.keywordPlus | MOLECULAR-WEIGHT | - |
dc.subject.keywordPlus | ACUTE TOXICITY | - |
dc.subject.keywordPlus | IN-SITU | - |
dc.subject.keywordPlus | POLYMER | - |
dc.subject.keywordAuthor | vascular endothelial growth factor receptor 1 targetingpeptide | - |
dc.subject.keywordAuthor | elastin-based polypeptides | - |
dc.subject.keywordAuthor | stimuli-responsiveness | - |
dc.subject.keywordAuthor | anti-angiogenesis | - |
dc.subject.keywordAuthor | neovascular diseases | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acsami.3c03989 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.