Biosynthesis of gold nanoparticles using marine microbe (Vibrio alginolyticus) and its anticancer and antioxidant analysis

Abstract

The applications of green synthesised gold nanoparticles for the biomedical field are the most prominent and developing area in this modern era. In this present investigation, marine bacteria, Vibrio alginolyticus was used for the biosynthesis of gold nanoparticles. The biosynthesized gold nanoparticles (AuNps) were characterized by various spectroscopic analysis. The morphological analysis of AuNps was done through scanning electron microscope (SEM) and transmission electron microscope (TEM) respectively. The obtained size was estimated around 100-150 nm. The synthesized AuNps confirmed by its surface plasmon resonance peak (SPR) range using UV-Vis spectrophotometer. The bio-metabolites from marine bacteria which was responsible for the nanoparticles synthesis were analyzed using fourier transform infrared spectrophotometer (FT-IR). The biomedical application of synthesized eco-friendly AuNps analysed by different method such as, the antioxidant property and free radical scavenging activity through DPPH (1, 1- diphenyl 2-picrylhyorazyl) and metal chelating assay. The anticancer activity of colon carcinoma was confirmed through MTT assay while the apoptotic mediated cell death observed through the fluorescence analysis. The biosynthesized AuNPs showed a dose dependent inhibitory effect on the growth of colon cancer cells. The IC50 was 15 mu g/mL, and the maximum inhibition of the cell death was (>75%) obtained when treating 25 mu g/mL. The apoptotic mediated cell death observed through nuclear condensation of the treated cells. The green synthesis of AuNPs emphases - to be cost effective and environmental friendly with anti-inflammatory and anti-cancer effects against colon cancer.