Single-cell characterization of CRISPR-modified transcript isoforms with nanopore sequencing
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Heon Seok | - |
dc.contributor.author | Grimes, Susan M. | - |
dc.contributor.author | Hooker, Anna C. | - |
dc.contributor.author | Lau, Billy T. | - |
dc.contributor.author | Ji, Hanlee P. | - |
dc.date.accessioned | 2023-09-11T01:46:09Z | - |
dc.date.available | 2023-09-11T01:46:09Z | - |
dc.date.created | 2023-07-21 | - |
dc.date.issued | 2021-12 | - |
dc.identifier.issn | 1474-760X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/190342 | - |
dc.description.abstract | We developed a single-cell approach to detect CRISPR-modified mRNA transcript structures. This method assesses how genetic variants at splicing sites and splicing factors contribute to alternative mRNA isoforms. We determine how alternative splicing is regulated by editing target exon-intron segments or splicing factors by CRISPR-Cas9 and their consequences on transcriptome profile. Our method combines long-read sequencing to characterize the transcript structure and short-read sequencing to match the single-cell gene expression profiles and gRNA sequence and therefore provides targeted genomic edits and transcript isoform structure detection at single-cell resolution. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.title | Single-cell characterization of CRISPR-modified transcript isoforms with nanopore sequencing | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Heon Seok | - |
dc.identifier.doi | 10.1186/s13059-021-02554-1 | - |
dc.identifier.scopusid | 2-s2.0-85120910393 | - |
dc.identifier.wosid | 000727334200004 | - |
dc.identifier.bibliographicCitation | GENOME BIOLOGY, v.22, no.1 | - |
dc.relation.isPartOf | GENOME BIOLOGY | - |
dc.citation.title | GENOME BIOLOGY | - |
dc.citation.volume | 22 | - |
dc.citation.number | 1 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.subject.keywordPlus | CD45 | - |
dc.subject.keywordPlus | CIRCUITS | - |
dc.subject.keywordAuthor | Single-cell long-read CRISPR screen | - |
dc.subject.keywordAuthor | Single-cell CRISPR screen | - |
dc.subject.keywordAuthor | Single-cell long-read sequencing | - |
dc.subject.keywordAuthor | Single-cell sequencing | - |
dc.subject.keywordAuthor | Alternative splicing | - |
dc.subject.keywordAuthor | Transcript isoform | - |
dc.identifier.url | https://genomebiology.biomedcentral.com/articles/10.1186/s13059-021-02554-1 | - |
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