C5 alpha secreted by tumor mesenchymal stem-like cells mediates resistance to 5-aminolevulinic acid-based photodynamic therapy against glioblastoma tumorspheres
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Junseong | - |
dc.contributor.author | Oh, Seung Jae | - |
dc.contributor.author | Shim, Jin-Kyoung | - |
dc.contributor.author | Ji, Young Bin | - |
dc.contributor.author | Moon, Ju Hyung | - |
dc.contributor.author | Kim, Eui Hyun | - |
dc.contributor.author | Huh, Yong-Min | - |
dc.contributor.author | Suh, Jin-Suck | - |
dc.contributor.author | Chang, Jong Hee | - |
dc.contributor.author | Lee, Su-Jae | - |
dc.contributor.author | Kang, Seok-Gu | - |
dc.date.accessioned | 2023-09-18T05:33:27Z | - |
dc.date.available | 2023-09-18T05:33:27Z | - |
dc.date.created | 2022-10-06 | - |
dc.date.issued | 2023-07 | - |
dc.identifier.issn | 0171-5216 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/190535 | - |
dc.description.abstract | Purpose Advancements in photodynamic diagnosis (PDD) and photodynamic therapy (PDT) as a standard care in cancer therapy have been limited. This study is aimed to investigate the clinical availability of 5-aminolevulinic acid (5-ALA)-based PDD and PDT in glioblastoma (GBM) patient-derived tumorspheres (TSs) and mouse orthotopic xenograft model. Methods PDT was performed using a 635 nm light-emitting diode (LED). Transcriptome profiles were obtained from microarray data. For knockdown of C5 alpha, siRNA was transfected into tumor mesenchymal stem-like cells (tMSLCs). The invasiveness of TSs was quantified using collagen-based 3D invasion assays. Results Treatment with 1 mM 5 ALA induced distinct protoporphyrin IX (PpIX) fluorescence in GBM TSs, but not in non-tumor cells or tissues, including tMSLCs. These observations were negatively correlated with the expression levels of FECH, which catalyzes the conversion of accumulated PpIX to heme. Furthermore, the 5-ALA-treated GBM TSs were sensitive to PDT, thereby significantly decreasing cell viability and invasiveness. Notably, the effects of PDT were abolished by culturing TSs with tMSLC-conditioned media. Transcriptome analysis revealed diverse tMSLC-secreted chemokines, including C5 alpha, and their correlations with the expression of stemness- or mesenchymal transition-associated genes. By adding or inhibiting C5 alpha, we confirmed that acquired resistance to PDT was induced via tMSLC-secreted C5 alpha. Conclusions Our results show substantial therapeutic effects of 5-ALA-based PDT on GBM TSs, suggesting C5 alpha as a key molecule responsible for PDT resistance. These findings could trigger PDT as a standard clinical modality for the treatment of GBM. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER | - |
dc.title | C5 alpha secreted by tumor mesenchymal stem-like cells mediates resistance to 5-aminolevulinic acid-based photodynamic therapy against glioblastoma tumorspheres | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Su-Jae | - |
dc.identifier.doi | 10.1007/s00432-022-04347-w | - |
dc.identifier.scopusid | 2-s2.0-85138073072 | - |
dc.identifier.wosid | 000854713300003 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, v.149, no.8, pp.4391 - 4402 | - |
dc.relation.isPartOf | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | - |
dc.citation.title | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | - |
dc.citation.volume | 149 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 4391 | - |
dc.citation.endPage | 4402 | - |
dc.type.rims | ART | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | PROTOPORPHYRIN-IX | - |
dc.subject.keywordPlus | ADJUVANT TEMOZOLOMIDE | - |
dc.subject.keywordPlus | FLUORESCENCE | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | RADIOTHERAPY | - |
dc.subject.keywordPlus | CONCOMITANT | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordAuthor | 5-Aminolevulilic acid | - |
dc.subject.keywordAuthor | Acquired PDT resistance | - |
dc.subject.keywordAuthor | Glioblastoma | - |
dc.subject.keywordAuthor | Photodynamic diagnosis | - |
dc.subject.keywordAuthor | Photodynamic therapy | - |
dc.subject.keywordAuthor | Tumor mesenchymal stem-like cells | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s00432-022-04347-w | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.