Intracellular synthesis of gold nanoparticles by Gluconacetobacter liquefaciens for delivery of peptide CopA3 and ginsenoside and anti-inflammatory effect on lipopolysaccharide-activated macrophages
DC Field | Value | Language |
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dc.contributor.author | Liu, Ying | - |
dc.contributor.author | PERUMALSAMY HARIBALAN | - |
dc.contributor.author | Kang, Chang Ho | - |
dc.contributor.author | Kim, Seung Hyun | - |
dc.contributor.author | Hwang, Jae-Sam | - |
dc.contributor.author | Koh, Sung-Cheol | - |
dc.contributor.author | Yi, Tae-Hoo | - |
dc.contributor.author | Kim, Yeon-Ju | - |
dc.date.accessioned | 2023-09-26T10:05:12Z | - |
dc.date.available | 2023-09-26T10:05:12Z | - |
dc.date.created | 2023-07-19 | - |
dc.date.issued | 2020-04 | - |
dc.identifier.issn | 2169-1401 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/191415 | - |
dc.description.abstract | Probiotic Gluconacetobacter strains are intestinal microbes with beneficial effects on human health. Recently, researchers have used these strains to biosynthesize metal and non-metal nanoparticles for treating various chronic diseases. Despite their importance in nanotechnology, gold nanoparticles (AuNPs) biosynthesized by Gluconacetobacter species have not been clearly identified for treating inflammation and inflammation-associated diseases. While ginsenoside CK has strong pharmaceutical activity, it also has strong cytotoxicity and hydrophobicity which is hurdle to make formulation. Peptide-nanoparticle hybrids are gaining increasing attention for their potential biomedical applications, including human inflammatory diseases. Herein, we developed peptide CopA3 surface conjugated and ginsenoside compound K (CK) loaded gold nanoparticles (GNP-CK-CopA3), which intracellularly synthesised by the probiotic Gluconacetobacter liquefaciens kh-1, to target lipopolysaccharide (LPS)-activated RAW264.7 macrophages. The synthetic GNP-CK-CopA3 was characterised by various instrumental techniques. The results of our cellular uptake and MTT assays exhibited obvious drug intracellular delivery without significant cytotoxicity. In addition, pre-treatment with GNP-CK-CopA3 significantly ameliorated LPS-induced nitric oxide (NO) and reactive oxygen species (ROS) production and suppressed the mRNA and protein expression of pro-inflammatory cytokines in macrophages. Furthermore, GNP-CK-CopA3 efficiently inhibited the activation of the nuclear factor-kappa B (NF-kappa B) and mitogen-activating protein kinase (MAPK) signalling pathways. Taken together, our findings highlight the potential of using peptide-nanoparticle hybrids in the development of anti-inflammatory approaches and providing the experimental foundation for further application. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.title | Intracellular synthesis of gold nanoparticles by Gluconacetobacter liquefaciens for delivery of peptide CopA3 and ginsenoside and anti-inflammatory effect on lipopolysaccharide-activated macrophages | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | PERUMALSAMY HARIBALAN | - |
dc.identifier.doi | 10.1080/21691401.2020.1748639 | - |
dc.identifier.scopusid | 2-s2.0-85083657238 | - |
dc.identifier.wosid | 000527159000001 | - |
dc.identifier.bibliographicCitation | ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, v.48, no.1, pp.777 - 788 | - |
dc.relation.isPartOf | ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | - |
dc.citation.title | ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | - |
dc.citation.volume | 48 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 777 | - |
dc.citation.endPage | 788 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | COMPOUND K | - |
dc.subject.keywordPlus | INFLAMMATORY RESPONSES | - |
dc.subject.keywordPlus | RAW264.7 CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | PATHWAYS | - |
dc.subject.keywordPlus | EXTRACT | - |
dc.subject.keywordAuthor | Peptide CopA3 | - |
dc.subject.keywordAuthor | Compound K | - |
dc.subject.keywordAuthor | gold nanoparticles | - |
dc.subject.keywordAuthor | anti-inflammatory | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.identifier.url | https://www.tandfonline.com/doi/full/10.1080/21691401.2020.1748639 | - |
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