Single-cell multi-gene identification of somatic mutations and gene rearrangements in cancer
DC Field | Value | Language |
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dc.contributor.author | Grimes, Susan M | - |
dc.contributor.author | Kim, Heon Seok | - |
dc.contributor.author | Roy, Sharmili | - |
dc.contributor.author | Sathe, Anuja | - |
dc.contributor.author | Ayala, Carlos I | - |
dc.contributor.author | Bai, Xiangqi | - |
dc.contributor.author | Almeda-Notestine, Alison F | - |
dc.contributor.author | Haebe, Sarah | - |
dc.contributor.author | Shree, Tanaya | - |
dc.contributor.author | Levy, Ronald | - |
dc.contributor.author | Lau, Billy T | - |
dc.contributor.author | Ji, Hanlee P | - |
dc.date.accessioned | 2023-10-10T02:32:24Z | - |
dc.date.available | 2023-10-10T02:32:24Z | - |
dc.date.created | 2023-10-07 | - |
dc.date.issued | 2023-07 | - |
dc.identifier.issn | 2632-8674 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/191764 | - |
dc.description.abstract | In this proof-of-concept study, we developed a single-cell method that provides genotypes of somatic alterations found in coding regions of messenger RNAs and integrates these transcript-based variants with their matching cell transcriptomes. We used nanopore adaptive sampling on single-cell complementary DNA libraries to validate coding variants in target gene transcripts, and short-read sequencing to characterize cell types harboring the mutations. CRISPR edits for 16 targets were identified using a cancer cell line, and known variants in the cell line were validated using a 352-gene panel. Variants in primary cancer samples were validated using target gene panels ranging from 161 to 529 genes. A gene rearrangement was also identified in one patient, with the rearrangement occurring in two distinct tumor sites. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Oxford University Press | - |
dc.title | Single-cell multi-gene identification of somatic mutations and gene rearrangements in cancer | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Heon Seok | - |
dc.identifier.doi | 10.1093/narcan/zcad034 | - |
dc.identifier.wosid | 001022568600004 | - |
dc.identifier.bibliographicCitation | NAR Cancer, v.5, no.3, pp.1 - 17 | - |
dc.relation.isPartOf | NAR Cancer | - |
dc.citation.title | NAR Cancer | - |
dc.citation.volume | 5 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 17 | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.subject.keywordPlus | REVEALS | - |
dc.identifier.url | https://academic.oup.com/narcancer/article/5/3/zcad034/7222080 | - |
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