The role of CK2 in the regulation of mitochondrial autophagy induced by rotenone
DC Field | Value | Language |
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dc.contributor.author | Kim, Beom Hee | - |
dc.contributor.author | Koh, Hyun Chul | - |
dc.date.accessioned | 2023-10-10T02:59:02Z | - |
dc.date.available | 2023-10-10T02:59:02Z | - |
dc.date.created | 2023-05-30 | - |
dc.date.issued | 2023-06 | - |
dc.identifier.issn | 0378-4274 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/191985 | - |
dc.description.abstract | CK2 regulates receptor-mediated mitophagy that removes damaged mitochondria. The PINK1/Parkin pathways also involve mitochondrial clearance through mitophagy. However, it is not clear whether CK2 regulates PINK1/Parkin-dependent mitophagy in response to stress. Rotenone treatment showed a decrease of FUNDC1 expression in the mitochondrial fraction of SH-SY5Y and HeLa cells, but an increase of PINK1/Parkin expression only in SH-SY5Y cells. Interestingly, CK2 inhibition increased mitochondrial LC3II expression in rotenone-treated HeLa cells, whereas it decreased in SH-SY5Y cells, indicating that CK2 mediates rotenone-induced mitophagy in dopaminergic neurons. Furthermore, FUNDC1 expression increased in rotenone-treated SH-SY5Y cells by CK2 inhibition, whereas it decreased in HeLa cells. CK2 inhibition also blocked the increase of Drp1, PINK1 and Parkin translocation into mitochondria and decrease of PGAM5 expression in rotenone-treated SH-SY5Y cells. As expected, rotenone treatment in PGAM5-knockdown cells reduced the expression of PINK1 and Parkin and decrease of LC3II expression. Interestingly, we observed that knockdown of CK2α or PGAM5 induced a further increase in caspase-3 expression. These results suggest that PINK1/Parkin-dependent mitophagy was dominant over FUNDC1 receptor-mediated mitophagy. Collectively, our findings suggest that CK2 can positively induce PINK1/Parkin-dependent mitophagy, and that mitophagy regulates cytoprotective effects by CK2 signaling in dopaminergic neurons. Data availability statement: All data generated or analyzed during this study are available upon request. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Ireland Ltd | - |
dc.title | The role of CK2 in the regulation of mitochondrial autophagy induced by rotenone | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Koh, Hyun Chul | - |
dc.identifier.doi | 10.1016/j.toxlet.2023.05.002 | - |
dc.identifier.scopusid | 2-s2.0-85159561128 | - |
dc.identifier.wosid | 001007074600001 | - |
dc.identifier.bibliographicCitation | Toxicology Letters, v.382, pp.1 - 12 | - |
dc.relation.isPartOf | Toxicology Letters | - |
dc.citation.title | Toxicology Letters | - |
dc.citation.volume | 382 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 12 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Toxicology | - |
dc.relation.journalWebOfScienceCategory | Toxicology | - |
dc.subject.keywordPlus | PINK1/PARKIN-MEDIATED MITOPHAGY | - |
dc.subject.keywordPlus | DAMAGED MITOCHONDRIA | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | PARKIN | - |
dc.subject.keywordPlus | PHOSPHORYLATION | - |
dc.subject.keywordPlus | PHOSPHATASE | - |
dc.subject.keywordPlus | FUNDC1 | - |
dc.subject.keywordPlus | DYNAMICS | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordAuthor | CK2 | - |
dc.subject.keywordAuthor | FUNDC1 | - |
dc.subject.keywordAuthor | Mitophagy | - |
dc.subject.keywordAuthor | PGAM5 | - |
dc.subject.keywordAuthor | PINK1 | - |
dc.subject.keywordAuthor | Parkin | - |
dc.subject.keywordAuthor | Rotenone | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0378427423001789?via%3Dihub | - |
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