Detection of Nα-terminally formylated native proteins by a pan-N-formyl methionine-specific antibody
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Dasom | - |
dc.contributor.author | Seok, Ok-Hee | - |
dc.contributor.author | Ju, Shinyeong | - |
dc.contributor.author | Kim, Sang-Yoon | - |
dc.contributor.author | Kim, Jeong-Mok | - |
dc.contributor.author | Lee, Cheolju | - |
dc.contributor.author | Hwang, Cheol-Sang | - |
dc.date.accessioned | 2023-10-10T03:05:42Z | - |
dc.date.available | 2023-10-10T03:05:42Z | - |
dc.date.created | 2023-05-03 | - |
dc.date.issued | 2023-05 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/192037 | - |
dc.description.abstract | N-formyl methionine (fMet)-containing proteins are produced in bacteria, eukaryotic organelles mitochondria and plastids, and even in cytosol. However, Nα-terminally formylated proteins have been poorly characterized because of the lack of appropriate tools to detect fMet independently of downstream proximal sequences. Using a fMet-Gly-Ser-Gly-Cys peptide as an antigen, we generated a pan-fMet-specific rabbit polyclonal antibody called anti-fMet. The raised anti-fMet recognized universally and sequence context–independently Nt-formylated proteins in bacterial, yeast, and human cells as determined by a peptide spot array, dot blotting, and immunoblotting. We anticipate that the anti-fMet antibody will be broadly used to enable an understanding of the poorly explored functions and mechanisms of Nt-formylated proteins in various organisms. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | American Society for Biochemistry and Molecular Biology Inc. | - |
dc.title | Detection of Nα-terminally formylated native proteins by a pan-N-formyl methionine-specific antibody | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jeong-Mok | - |
dc.identifier.doi | 10.1016/j.jbc.2023.104652 | - |
dc.identifier.scopusid | 2-s2.0-85153089298 | - |
dc.identifier.wosid | 001009526700001 | - |
dc.identifier.bibliographicCitation | Journal of Biological Chemistry, v.299, no.5, pp.1 - 11 | - |
dc.relation.isPartOf | Journal of Biological Chemistry | - |
dc.citation.title | Journal of Biological Chemistry | - |
dc.citation.volume | 299 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 11 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | PEPTIDE DEFORMYLASE | - |
dc.subject.keywordPlus | TRANSFER-RNA | - |
dc.subject.keywordPlus | SOLUBLE-RNA | - |
dc.subject.keywordPlus | RECEPTORS | - |
dc.subject.keywordPlus | INITIATOR | - |
dc.subject.keywordPlus | FORMYLTRANSFERASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | RIBOSOME | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordAuthor | anti-fMet antibody | - |
dc.subject.keywordAuthor | fMet/N-degron | - |
dc.subject.keywordAuthor | mitochondrial methionyl-tRNA formyltransferase | - |
dc.subject.keywordAuthor | N-formyl methionine | - |
dc.subject.keywordAuthor | peptide deformylase | - |
dc.subject.keywordAuthor | protein synthesis | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0021925823002946?via%3Dihub | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.