Deubiquitinase USP19 enhances phenylalanine hydroxylase protein stability and its enzymatic activity
- Authors
- Sarodaya, Neha; Tyagi, Apoorvi; Kim, Hyun-Jin; Colaco, Jencia Carminha; Kang, Ju-Seop; Kim, Woo Jin; Kim, Kye-Seong; Ramakrishna, Suresh
- Issue Date
- Oct-2023
- Publisher
- SPRINGER
- Keywords
- Homeostasis; Hyperphenylalaninemia; Metabolic activity; Proteolysis; Tetrahydrobiopterin; Ubiquitin proteasome system
- Citation
- CELL BIOLOGY AND TOXICOLOGY, v.39, no.5, pp.2295 - 2310
- Indexed
- SCIE
SCOPUS
- Journal Title
- CELL BIOLOGY AND TOXICOLOGY
- Volume
- 39
- Number
- 5
- Start Page
- 2295
- End Page
- 2310
- URI
- https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/192095
- DOI
- 10.1007/s10565-022-09719-z
- ISSN
- 0742-2091
- Abstract
- Phenylalanine hydroxylase (PAH) is the key enzyme in phenylalanine metabolism, deficiency of which is associated with the most common metabolic phenotype of phenylketonuria (PKU) and hyperphenylalaninemia (HPA). A bulk of PKU disease-associated missense mutations in the PAH gene have been studied, and the consequence of each PAH variant vary immensely. Prior research established that PKU-associated variants possess defects in protein folding with reduced cellular stability leading to rapid degradation. However, recent evidence revealed that PAH tetramers exist as a mixture of resting state and activated state whose transition depends upon the phenylalanine concentration and certain PAH variants that fail to modulate the structural equilibrium are associated with PKU disease. Collectively, these findings framed our understanding of the complex genotype-phenotype correlation in PKU. In the current study, we substantiate a link between PAH protein stability and its degradation by the ubiquitin-mediated proteasomal degradation system. Here, we provide an evidence that PAH protein undergoes ubiquitination and proteasomal degradation, which can be reversed by deubiquitinating enzymes (DUBS). We identified USP19 as a novel DUB that regulates PAH protein stability. We found that ectopic expression of USP19 increased PAH protein level, whereas depletion of USP19 promoted PAH protein degradation. Our study indicates that USP19 interacts with PAH and prevents polyubiquitination of PAH subsequently extending the half-life of PAH protein. Finally, the increase in the level of PAH protein by the deubiquitinating activity of USP19 resulted in enhanced metabolic function of PAH. In summary, our study identifies the role of USP19 in regulating PAH protein stability and promotes its metabolic activity.
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