Effects of sitagliptin on peritoneal membrane: The potential role of mesothelial cell tight junction proteins
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jo, Chor Ho | - |
dc.contributor.author | Kim, Sua | - |
dc.contributor.author | Ha, Tae Kyung | - |
dc.contributor.author | Kang, Duk-Hee | - |
dc.contributor.author | Kim, Gheun-Ho | - |
dc.date.accessioned | 2023-11-24T02:30:39Z | - |
dc.date.available | 2023-11-24T02:30:39Z | - |
dc.date.created | 2023-05-03 | - |
dc.date.issued | 2023-11 | - |
dc.identifier.issn | 0896-8608 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/192851 | - |
dc.description.abstract | Background: The roles of tight junction (TJ) proteins in peritoneal membrane transport and peritoneal dialysis (PD) require further characterisation. Dipeptidyl peptidase-4 is expressed in mesothelial cells, and its activity may affect peritoneal membrane function and morphology. Methods: Human peritoneal mesothelial cells (HPMCs) were isolated and cultured from omentum obtained during abdominal surgery, and paracellular transport functions were evaluated by measuring transmesothelial electrical resistance (TMER) and dextran flux. Sprague-Dawley rats were infused daily with 4.25% peritoneal dialysate with and without sitagliptin administration for 8 weeks. At the end of this period, rat peritoneal mesothelial cells (RPMCs) were isolated to evaluate TJ protein expression. Results: In HPMCs, the protein expression of claudin-1, claudin-15, occludin and E-cadherin was decreased by TGF-beta treatment but reversed by sitagliptin co-treatment. TMER was decreased by TGF-beta treatment but improved by sitagliptin co-treatment. Consistent with this, dextran flux was increased by TGF-beta treatment and reversed by sitagliptin co-treatment. In the animal experiment, sitagliptin-treated rats had a lower D2/D0 glucose ratio and a higher D2/P2 creatinine ratio than PD controls during the peritoneal equilibration test. Protein expression of claudin-1, claudin-15 and E-cadherin decreased in RPMCs from PD controls but was not affected in those from sitagliptin-treated rats. Peritoneal fibrosis was induced in PD controls but ameliorated in sitagliptin-treated rats. Conclusion: The expression of TJ proteins including claudin-1 and claudin-15 was associated with transport function both in HPMCs and in a rat model of PD. Sitagliptin prevents peritoneal fibrosis in PD and can potentially restore peritoneal mesothelial cell TJ proteins. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SAGE PUBLICATIONS INC | - |
dc.title | Effects of sitagliptin on peritoneal membrane: The potential role of mesothelial cell tight junction proteins | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Ha, Tae Kyung | - |
dc.contributor.affiliatedAuthor | Kim, Gheun-Ho | - |
dc.identifier.doi | 10.1177/08968608231158224 | - |
dc.identifier.scopusid | 2-s2.0-85152543952 | - |
dc.identifier.wosid | 000960749100001 | - |
dc.identifier.bibliographicCitation | PERITONEAL DIALYSIS INTERNATIONAL, v.43, no.6, pp.448 - 456 | - |
dc.relation.isPartOf | PERITONEAL DIALYSIS INTERNATIONAL | - |
dc.citation.title | PERITONEAL DIALYSIS INTERNATIONAL | - |
dc.citation.volume | 43 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 448 | - |
dc.citation.endPage | 456 | - |
dc.type.rims | ART | - |
dc.type.docType | Article; Early Access | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Urology & Nephrology | - |
dc.relation.journalWebOfScienceCategory | Urology & Nephrology | - |
dc.subject.keywordPlus | LONGITUDINAL CHANGES | - |
dc.subject.keywordPlus | DIALYSIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | FIBROSIS | - |
dc.subject.keywordPlus | WATER | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | TRANSPORT | - |
dc.subject.keywordPlus | FAILURE | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordAuthor | Claudin-1 | - |
dc.subject.keywordAuthor | claudin-15 | - |
dc.subject.keywordAuthor | E-cadherin | - |
dc.subject.keywordAuthor | mesothelial cell | - |
dc.subject.keywordAuthor | peritoneal transport | - |
dc.subject.keywordAuthor | sitagliptin | - |
dc.identifier.url | https://journals.sagepub.com/doi/10.1177/08968608231158224 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.