Photosensitizing deep-seated cancer cells with photoprotein-conjugated upconversion nanoparticles
DC Field | Value | Language |
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dc.contributor.author | Park, Sung Hyun | - |
dc.contributor.author | Han, Soohyun | - |
dc.contributor.author | Park, Sangwoo | - |
dc.contributor.author | Kim, Hyung Shik | - |
dc.contributor.author | Kim, Kyung-Min | - |
dc.contributor.author | Kim, Suyeon | - |
dc.contributor.author | Lee, Dong Yun | - |
dc.contributor.author | Lee, Joonseok | - |
dc.contributor.author | Kim, Young-Pil | - |
dc.date.accessioned | 2023-11-24T04:51:11Z | - |
dc.date.available | 2023-11-24T04:51:11Z | - |
dc.date.issued | 2023-08 | - |
dc.identifier.issn | 1477-3155 | - |
dc.identifier.issn | 1477-3155 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/192956 | - |
dc.description.abstract | To resolve the problem of target specificity and light transmission to deep-seated tissues in photodynamic therapy (PDT), we report a cancer cell-targeted photosensitizer using photoprotein-conjugated upconversion nanoparticles (UCNPs) with high target specificity and efficient light transmission to deep tissues. Core-shell UCNPs with low internal energy back transfer were conjugated with recombinant proteins that consists of a photosensitizer (KillerRed; KR) and a cancer cell-targeted lead peptide (LP). Under near infrared (NIR)-irradiating condition, the UCNP-KR-LP generated superoxide anion radicals as reactive oxygen species via NIR-to-green light conversion and exhibited excellent specificity to target cancer cells through receptor-mediated cell adhesion. Consequently, this photosensitizing process facilitated rapid cell death in cancer cell lines (MCF-7, MDA-MB-231, and U-87MG) overexpressing integrin beta 1 (ITGB1) receptors but not in a cell line (SK-BR-3) with reduced ITGB1 expression and a non-invasive normal breast cell line (MCF-10A). In contrast to green light irradiation, NIR light irradiation exhibited significant PDT efficacy in cancer cells located beneath porcine skin tissues up to a depth of 10 mm, as well as in vivo tumor xenograft mouse models. This finding suggests that the designed nanocomposite is useful for sensing and targeting various deep-seated tumors. | - |
dc.format.extent | 17 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | BioMed Central Ltd | - |
dc.title | Photosensitizing deep-seated cancer cells with photoprotein-conjugated upconversion nanoparticles | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1186/s12951-023-02057-0 | - |
dc.identifier.scopusid | 2-s2.0-85168393417 | - |
dc.identifier.wosid | 001051545700003 | - |
dc.identifier.bibliographicCitation | Journal of Nanobiotechnology, v.21, no.1, pp 1 - 17 | - |
dc.citation.title | Journal of Nanobiotechnology | - |
dc.citation.volume | 21 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 17 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | VIVO PHOTODYNAMIC THERAPY | - |
dc.subject.keywordPlus | LASER-LIGHT | - |
dc.subject.keywordPlus | PENETRATION | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | OXYGEN | - |
dc.subject.keywordAuthor | Photodynamic therapy | - |
dc.subject.keywordAuthor | Protein photosensitizers | - |
dc.subject.keywordAuthor | Upconversion nanoparticles | - |
dc.subject.keywordAuthor | Reactive oxygen species | - |
dc.subject.keywordAuthor | Near-infrared light | - |
dc.identifier.url | https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-023-02057-0 | - |
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