Feasibility of Raman spectroscopic identification of gall bladder cancer using extracellular vesicles extracted from bile
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bui, Thu Thuy | - |
dc.contributor.author | Jang, Eunjin | - |
dc.contributor.author | Shin, Ji Hyun | - |
dc.contributor.author | Kim, Tae Hun | - |
dc.contributor.author | Kim, Hayoon | - |
dc.contributor.author | Choi, Dongho | - |
dc.contributor.author | Vu, Tung Duy | - |
dc.contributor.author | Chung, Hoeil | - |
dc.date.accessioned | 2023-11-24T04:53:09Z | - |
dc.date.available | 2023-11-24T04:53:09Z | - |
dc.date.created | 2023-08-21 | - |
dc.date.issued | 2023-07 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/192965 | - |
dc.description.abstract | Extracellular vesicles (EVs), which are heterogeneous membrane-based vesicles with bilayer cell membrane structures, could be versatile biomarkers for the identification of diverse diseases including cancers. With this potential, this study has attempted the Raman spectroscopic identification of gall bladder (GB) cancer by directly measuring the EV solution extracted from human bile without further sample drying. For this purpose, bile samples were obtained from four normal individuals and 21 GB polyp, eight hepatocellular carcinoma (HCC), and five GB cancer patients, and EVs were extracted from each of the bile samples. The Raman peak shapes of the EVs extracted from the GB cancer samples, especially the relative intensities of peaks in the 1560-1340 cm−1 range, were dissimilar to those of the samples from the normal, GB polyp, and HCC groups. The intensity ratios of peaks at 1537 and 1453 cm−1 and at 1395 and 1359 cm−1 of the GB cancer samples were lower and higher, respectively, than those of the samples of the remaining three groups. The differences of peak intensity ratios were statistically significant based on the Mann-Whitney U test. DNA/RNA bases, amino acids, and bile salts contributed to the spectra of EVs, and their relative abundances seemed to vary according to the occurrence of GB cancer. The varied metabolite compositions and/or structures of EVs were successfully demonstrated by the dissimilar peak intensity ratios in the Raman spectra, thereby enabling the discrimination of GB cancer. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Royal Society of Chemistry | - |
dc.title | Feasibility of Raman spectroscopic identification of gall bladder cancer using extracellular vesicles extracted from bile | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Dongho | - |
dc.contributor.affiliatedAuthor | Chung, Hoeil | - |
dc.identifier.doi | 10.1039/d3an00806a | - |
dc.identifier.scopusid | 2-s2.0-85167424847 | - |
dc.identifier.wosid | 001037422500001 | - |
dc.identifier.bibliographicCitation | Analyst, v.148, no.17, pp.4156 - 4165 | - |
dc.relation.isPartOf | Analyst | - |
dc.citation.title | Analyst | - |
dc.citation.volume | 148 | - |
dc.citation.number | 17 | - |
dc.citation.startPage | 4156 | - |
dc.citation.endPage | 4165 | - |
dc.type.rims | ART | - |
dc.type.docType | Article in press | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | Cytology | - |
dc.subject.keywordPlus | Diseases | - |
dc.subject.keywordPlus | Extraction | - |
dc.subject.keywordPlus | Raman spectroscopy | - |
dc.subject.keywordPlus | Salts | - |
dc.subject.keywordPlus | Bi-layer | - |
dc.subject.keywordPlus | Bladder cancers | - |
dc.subject.keywordPlus | Extracellular | - |
dc.subject.keywordPlus | Gall bladder | - |
dc.subject.keywordPlus | Hepatocellular carcinoma | - |
dc.subject.keywordPlus | Heterogeneous membranes | - |
dc.subject.keywordPlus | Membrane-based | - |
dc.subject.keywordPlus | Peak intensity ratio | - |
dc.subject.keywordPlus | Raman spectroscopic | - |
dc.subject.keywordPlus | Spectroscopic identification | - |
dc.subject.keywordPlus | Metabolites | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2023/AN/D3AN00806A | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.