Molecular characterization of azoreductase and its potential for the decolorization of Remazol Red R and Acid Blue 29
DC Field | Value | Language |
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dc.contributor.author | Mustafa, Ghulam | - |
dc.contributor.author | Zahid, Muhammad Tariq | - |
dc.contributor.author | Bharat Kurade, Mayur | - |
dc.contributor.author | Mahadeo Patil, Swapnil | - |
dc.contributor.author | Shakoori, Farah Rauf | - |
dc.contributor.author | Shafiq, Zeeshan | - |
dc.contributor.author | Ihsan, Sidra | - |
dc.contributor.author | Ahn, Yongtae | - |
dc.contributor.author | Khan, Azmat Ali | - |
dc.contributor.author | Jeon, Byong-hun | - |
dc.contributor.author | Gacem, Amel | - |
dc.date.accessioned | 2023-11-24T04:54:01Z | - |
dc.date.available | 2023-11-24T04:54:01Z | - |
dc.date.created | 2023-08-17 | - |
dc.date.issued | 2023-10 | - |
dc.identifier.issn | 0269-7491 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/192970 | - |
dc.description.abstract | Azoreductase is a reductive enzyme that efficiently biotransformed textile azo dyes. This study demonstrated the heterologous overexpression of the azoreductase gene in Escherichia coli for the effective degradation of Remazol Red-R and Acid-Blue 29 dyes. The AzK gene of Klebsiella pneumoniae encoding a ≈22 kDa azoreductase enzyme was cloned into the pET21+C expression vector. The inoculum size of 1.5%, IPTG concentration of 0.5 mM, and incubation time of 6 h were optimized by response surface methodology a statistical tool. The crude extract showed 76% and 74%, while the purified enzyme achieved 94% and 93% decolorization of RRR and AB-29, respectively in 0.3 h. The reaction kinetics showed that RRR had a Km and Vmax value of 0.058 mM and 1416 U mg−1, respectively at an NADH concentration of 10 mM. HPLC and GC-MS analyses showed that RRR was effectively bio-transformed by azoreductase to 2-[3-(hydroxy-amino) benzene-1-sulfonyl and AB-29 to aniline and 3-nitrosoaniline. This study explored the potential of recombinant azoreductase isolated from K. pneumoniae in the degradation of toxic textile azo dyes into less toxic metabolites. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Elsevier Ltd | - |
dc.title | Molecular characterization of azoreductase and its potential for the decolorization of Remazol Red R and Acid Blue 29 | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Jeon, Byong-hun | - |
dc.identifier.doi | 10.1016/j.envpol.2023.122253 | - |
dc.identifier.scopusid | 2-s2.0-85166197018 | - |
dc.identifier.wosid | 001055022000001 | - |
dc.identifier.bibliographicCitation | ENVIRONMENTAL POLLUTION, v.335, pp.1 - 11 | - |
dc.relation.isPartOf | ENVIRONMENTAL POLLUTION | - |
dc.citation.title | ENVIRONMENTAL POLLUTION | - |
dc.citation.volume | 335 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 11 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Environmental Sciences & Ecology | - |
dc.relation.journalWebOfScienceCategory | Environmental Sciences | - |
dc.subject.keywordPlus | OXYGEN-INSENSITIVE AZOREDUCTASE | - |
dc.subject.keywordPlus | AZO DYES | - |
dc.subject.keywordPlus | BREVIBACILLUS-LATEROSPORUS | - |
dc.subject.keywordPlus | TEXTILE-INDUSTRY | - |
dc.subject.keywordPlus | WASTE-WATER | - |
dc.subject.keywordPlus | BIODEGRADATION | - |
dc.subject.keywordPlus | DETOXIFICATION | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordPlus | EFFLUENT | - |
dc.subject.keywordPlus | DECOLOURIZATION | - |
dc.subject.keywordAuthor | Decolorization | - |
dc.subject.keywordAuthor | Remazol red | - |
dc.subject.keywordAuthor | Azoreductase | - |
dc.subject.keywordAuthor | Biodegradation | - |
dc.subject.keywordAuthor | Bioremediation | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0269749123012551?via%3Dihub | - |
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