iNrich, Rapid and Robust Method to Enrich N-Terminal Proteome in a Highly Multiplexed Platform
DC Field | Value | Language |
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dc.contributor.author | Ju, Shinyeong | - |
dc.contributor.author | Kwon, Yumi | - |
dc.contributor.author | Kim, Jeong-Mok | - |
dc.contributor.author | Park, Daechan | - |
dc.contributor.author | Lee, Seonjeong | - |
dc.contributor.author | Lee, Jin-Won | - |
dc.contributor.author | Hwang, Cheol-Sang | - |
dc.contributor.author | Lee, Cheolju | - |
dc.date.accessioned | 2021-07-30T04:53:42Z | - |
dc.date.available | 2021-07-30T04:53:42Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2020-05 | - |
dc.identifier.issn | 0003-2700 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/1935 | - |
dc.description.abstract | The field of terminal proteomics is limited in that it is optimized for large-scale analysis via multistep processes involving liquid chromatography. Here, we present an integrated N-terminal peptide enrichment method (iNrich) that can handle as little as 25 mu g of cell lysate via a single-stage encapsulated solid-phase extraction column. iNrich enables simple, rapid, and reproducible sample processing, treatment of a wide range of protein amounts (25 mu g similar to 1 mg), multiplexed parallel sample preparation, and in-stage sample prefractionation using a mixed-anion-exchange filter. We identified similar to 5000 N-terminal peptides (Nt-peptides) from only 100 mu g of human cell lysate including Nt-formyl peptides. Multiplexed sample preparation facilitated quantitative and robust enrichment of N-terminome iNrich with dozens of samples simultaneously. We further developed the method to incorporate isobaric tags such as a tandem mass tag (TMT) and used it to discover novel peptides during ER stress analysis. The iNrich facilitated high-throughput N-terminomics and degradomics at a low cost using commercially available reagents and apparatus, without requiring arduous procedures. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title | iNrich, Rapid and Robust Method to Enrich N-Terminal Proteome in a Highly Multiplexed Platform | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Jeong-Mok | - |
dc.contributor.affiliatedAuthor | Lee, Jin-Won | - |
dc.identifier.doi | 10.1021/acs.analchem.9b05653 | - |
dc.identifier.scopusid | 2-s2.0-85084835014 | - |
dc.identifier.wosid | 000530658600040 | - |
dc.identifier.bibliographicCitation | ANALYTICAL CHEMISTRY, v.92, no.9, pp.6462 - 6469 | - |
dc.relation.isPartOf | ANALYTICAL CHEMISTRY | - |
dc.citation.title | ANALYTICAL CHEMISTRY | - |
dc.citation.volume | 92 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 6462 | - |
dc.citation.endPage | 6469 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.subject.keywordPlus | PROTEOLYTIC EVENTS | - |
dc.subject.keywordPlus | PEPTIDES | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | TERMINOMICS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | PROJECT | - |
dc.subject.keywordPlus | TAILS | - |
dc.identifier.url | https://pubs.acs.org/doi/10.1021/acs.analchem.9b05653 | - |
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