Syntrophic bacteria- and Methanosarcina-rich acclimatized microbiota with better carbohydrate metabolism enhances biomethanation of fractionated lignocellulosic biocomponents

Abstract

An inadequate lignocellulolytic capacity of a conventional anaerobic digester sludge (ADS) microbiota is the bottleneck for the maximal utilization of lignocellulose in anaerobic digestion. A well-constructed microbial consortium acclimatized to lignocellulose outperformed the ADS in terms of biogas productivity when fractionated biocomponents of rice straw were used to achieve a high methane bioconversion rate. A 33.3 % higher methane yield was obtained with the acclimatized consortium (AC) compared to that of ADS control. The dominant pair-wise link between Firmicutes (18.99–40.03 %), Bacteroidota (10.94–28.75 %), and archaeal Halobacteriota (3.59–20.57 %) phyla in the AC seed digesters indicated that the keystone members of these phyla were responsible for higher methane yield. A high abundance of syntrophic bacteria such as Proteiniphilum (1.22–5.19 %), Fermentimonas (0.71–5.31 %), Syntrophomonas (0.87–3.59 %), and their syntrophic partner Methanosarcina (4.26–18.80 %) maintained the digester stability and facilitated higher substrate-to-methane conversion in the AC seed digesters. The present combined strategy will help in boosting the ‘biomass-to-methane” conversion.