Competent antigen-presenting cells are generated from the long-term culture of splenocytes with granulocyte-macrophage colony-stimulating factor
DC Field | Value | Language |
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dc.contributor.author | Ryu, Seul Hye | - |
dc.contributor.author | Na, Hye Young | - |
dc.contributor.author | Sohn, Moah | - |
dc.contributor.author | Choi, Wanho | - |
dc.contributor.author | In, Hyunju | - |
dc.contributor.author | Shin, Hyun Soo | - |
dc.contributor.author | Choi, Jae-Hoon | - |
dc.contributor.author | Park, Chae Gyu | - |
dc.date.accessioned | 2021-08-02T14:52:24Z | - |
dc.date.available | 2021-08-02T14:52:24Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2017-08 | - |
dc.identifier.issn | 0165-2478 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/19500 | - |
dc.description.abstract | Dendritic cells (DCs) are routinely produced from the culture of mouse bone marrow (BM) with granulocyte macrophage colony-stimulating factor (GM-CSF) within a period of 10 days. Although splenic extramedullary myelopoiesis was suggested to occur under the influence of GM-CSF, the hematopoietic outcome of splenic culture with GM-CSF has not been scrutinized. We have cultured mouse splenocytes with GM-CSF for an extended period of time, where we discovered that the CD11b(+)CD11c(+) cells began to proliferate prominently after 10 days and their number increased until the 4th week of the culture. In parallel experiments, FMS-like tyrosine kinase 3 (FLT3) and its ligand, FLT3L, were not found to influence the culture of splenocytes. Like DCs in the culture of BM with GM-CSF, a distinct population of CD11b(+)CD11c(+)MHC IIhi cells was readily identified as DCs in the long-term culture of splenocytes. After being isolated and plated overnight the CD11b(+)CD11c(+)MHC Il(hi) cells exhibited non-adherent dendritic morphology, while the other CD11b(+)CD11c(+) cells became adherent. Besides, these CD11b(+)CD11c(+)MHC IIhi cells possessed relatively weak endocytic and phagocytic abilities but displayed strong antigen-presenting capacities, revealing DC-like characteristics; in contrast, the other CD11b(+)CD11c(+) cells showed strong endocytosis and phagocytosis of antigens but were poor at antigen presentation, indicating macrophage-like traits. Therefore, we demonstrated that phenotypically as well as functionally genuine DCs are generated in the long-term culture of splenocytes with GM-CSF. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Competent antigen-presenting cells are generated from the long-term culture of splenocytes with granulocyte-macrophage colony-stimulating factor | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Jae-Hoon | - |
dc.identifier.doi | 10.1016/j.imlet.2017.06.010 | - |
dc.identifier.scopusid | 2-s2.0-85021983268 | - |
dc.identifier.wosid | 000407184100015 | - |
dc.identifier.bibliographicCitation | IMMUNOLOGY LETTERS, v.188, pp.96 - 107 | - |
dc.relation.isPartOf | IMMUNOLOGY LETTERS | - |
dc.citation.title | IMMUNOLOGY LETTERS | - |
dc.citation.volume | 188 | - |
dc.citation.startPage | 96 | - |
dc.citation.endPage | 107 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Immunology | - |
dc.relation.journalWebOfScienceCategory | Immunology | - |
dc.subject.keywordPlus | MOUSE BONE-MARROW | - |
dc.subject.keywordPlus | DENDRITIC CELLS | - |
dc.subject.keywordPlus | EXTRAMEDULLARY HEMATOPOIESIS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | GM-CSF | - |
dc.subject.keywordPlus | SPLEEN | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | PROGENITORS | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | PROTECT | - |
dc.subject.keywordAuthor | Antigen-presenting cells | - |
dc.subject.keywordAuthor | Dendritic cells | - |
dc.subject.keywordAuthor | GM-CSF | - |
dc.subject.keywordAuthor | Hematopoiesis | - |
dc.subject.keywordAuthor | Spleen | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0165247817300834?via%3Dihub | - |
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