Graying the self-assembly of gold nanoparticles for improved enzyme activity assays
DC Field | Value | Language |
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dc.contributor.author | Kim, Gae Baik | - |
dc.contributor.author | Lee, Jin Oh | - |
dc.contributor.author | Kim, Young-Pil | - |
dc.date.accessioned | 2021-08-02T14:53:17Z | - |
dc.date.available | 2021-08-02T14:53:17Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2017-07 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/19562 | - |
dc.description.abstract | Despite the simplicity and usability of gold nanoparticle (AuNP)-based colorimetry in a large panel of bioassays, this technique still suffers from a poor detection limit. To overcome this issue, we report an improved AuNP-based colorimetric assay in combination with simple centrifugation and silver enhancement. As a proof-of-concept, two types of enzymes (i.e., protein phosphatase and protease) were constructed based on the self-assembly of thiol-stabilized AuNPs in the presence of peptides and metal ions. When the AuNP solutions were subjected to a short cycle of centrifugation and silver enhancement, the signal-to-background ratio (SBR) was distinctly improved by a factor of 5–10 in both enzyme activity assays, as compared to that of AuNP-based colorimetry. The detection limit achieved by using the silver enhancement was determined to be improved by a factor of 1.0–3.4. Furthermore, grayscale images of the silver enhancement allowed for a rapid and simple enzyme assay that did not require measuring the absorbance. Due to its ability to improve the detection senstivity in a facile way, we anticipate that this approach will be suitable for use in many AuNP colorimetric assays. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE SA | - |
dc.title | Graying the self-assembly of gold nanoparticles for improved enzyme activity assays | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Kim, Young-Pil | - |
dc.identifier.doi | 10.1016/j.snb.2017.02.067 | - |
dc.identifier.scopusid | 2-s2.0-85013157712 | - |
dc.identifier.wosid | 000400803700033 | - |
dc.identifier.bibliographicCitation | SENSORS AND ACTUATORS B-CHEMICAL, v.246, pp.271 - 277 | - |
dc.relation.isPartOf | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.citation.title | SENSORS AND ACTUATORS B-CHEMICAL | - |
dc.citation.volume | 246 | - |
dc.citation.startPage | 271 | - |
dc.citation.endPage | 277 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Electrochemistry | - |
dc.relation.journalResearchArea | Instruments & Instrumentation | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Electrochemistry | - |
dc.relation.journalWebOfScienceCategory | Instruments & Instrumentation | - |
dc.subject.keywordPlus | ENHANCED RAMAN-SPECTROSCOPY | - |
dc.subject.keywordPlus | SILVER ENHANCEMENT | - |
dc.subject.keywordPlus | COLORIMETRIC DETECTION | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordPlus | IMMUNOASSAY | - |
dc.subject.keywordPlus | NANOSENSORS | - |
dc.subject.keywordPlus | SENSORS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordAuthor | Gold nanoparticle | - |
dc.subject.keywordAuthor | Silver enhancement | - |
dc.subject.keywordAuthor | Signal amplification | - |
dc.subject.keywordAuthor | Self-assembly | - |
dc.subject.keywordAuthor | Enzyme activity | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0925400517302940?via%3Dihub | - |
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