Micropatterning of proteins on ion beam-induced poly(acrylic acid)-grafted polyethylene film

Abstract

Micropatterns of proteins were created by using patterned ion beam irradiation onto a polyethylene film and graft polymerization of acrylic acid. Acrylic acid was selectively graft polymerized on the irradiated regions. The results of the grafting study revealed that the optimum fluence to achieve the maximum grafting degree was 1 x 10(15) ions/cm(2). Biotin was covalently immobilized on the grafted regions of the polyethylene film. Protein patterning was achieved through specific binding of biotin and streptavidin. The resolved protein patterns with the maximum fluorescence intensity were achieved on the poly(acrylic acid) (PAA)-grafted polyethylene films prepared at the fluence of 1 x 10(15) ions/cm(2). This method can be used for patterning of various biomolecules and for further biological applications.