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Exploring the dynamic nature of divalent metal ions involved in DNA cleavage by CRISPR-Cas12a
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| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Son, Heyjin | - |
| dc.contributor.author | Park, Jaeil | - |
| dc.contributor.author | Choi, You Hee | - |
| dc.contributor.author | Jung, Youngri | - |
| dc.contributor.author | Lee, Joong-Wook | - |
| dc.contributor.author | Bae, Sangsu | - |
| dc.contributor.author | Lee, Sanghwa | - |
| dc.date.accessioned | 2024-12-20T06:25:49Z | - |
| dc.date.available | 2024-12-20T06:25:49Z | - |
| dc.date.issued | 2022-02 | - |
| dc.identifier.issn | 1359-7345 | - |
| dc.identifier.issn | 1364-548X | - |
| dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/202807 | - |
| dc.description.abstract | CRISPR-Cas12a has been widely used in genome editing and nucleic acid detection. In both of these applications, Cas12a cleaves target DNA in a divalent metal ion-dependent manner. However, when and how metal ions contribute to the cleavage reaction is unclear. Here, using a single-molecule FRET assay, we reveal that these metal ions are necessary for stabilising cleavage-competent conformations and that they are easily exchangeable, suggesting that they are dynamically coordinated. | - |
| dc.format.extent | 4 | - |
| dc.language | 영어 | - |
| dc.language.iso | ENG | - |
| dc.publisher | ROYAL SOC CHEMISTRY | - |
| dc.title | Exploring the dynamic nature of divalent metal ions involved in DNA cleavage by CRISPR-Cas12a | - |
| dc.type | Article | - |
| dc.publisher.location | 영국 | - |
| dc.identifier.doi | 10.1039/d1cc04446j | - |
| dc.identifier.scopusid | 2-s2.0-85124437603 | - |
| dc.identifier.wosid | 000744134300001 | - |
| dc.identifier.bibliographicCitation | CHEMICAL COMMUNICATIONS, v.58, no.12, pp 1978 - 1981 | - |
| dc.citation.title | CHEMICAL COMMUNICATIONS | - |
| dc.citation.volume | 58 | - |
| dc.citation.number | 12 | - |
| dc.citation.startPage | 1978 | - |
| dc.citation.endPage | 1981 | - |
| dc.type.docType | Article | - |
| dc.description.isOpenAccess | Y | - |
| dc.description.journalRegisteredClass | scie | - |
| dc.description.journalRegisteredClass | scopus | - |
| dc.relation.journalResearchArea | Chemistry | - |
| dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
| dc.subject.keywordPlus | RNA-GUIDED ENDONUCLEASE | - |
| dc.subject.keywordPlus | CPF1 | - |
| dc.subject.keywordPlus | CATALYSIS | - |
| dc.subject.keywordPlus | CAS12A | - |
| dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2022/CC/D1CC04446J | - |
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Collections - 서울 자연과학대학 > 서울 화학과 > 1. Journal Articles
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