Post-transcriptional and translational regulation of mRNA-like long non-coding RNAs by microRNAs in early developmental stages of zebrafish embryos
DC Field | Value | Language |
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dc.contributor.author | Lee, Kyung-Tae | - |
dc.contributor.author | Nam, Jin-Wu | - |
dc.date.accessioned | 2021-08-02T15:29:04Z | - |
dc.date.available | 2021-08-02T15:29:04Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2017-04 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/20440 | - |
dc.description.abstract | At the post-transcriptional and translational levels, microRNA (miRNA) represses protein-coding genes via seed pairing to the 3' untranslated regions (UTRs) of mRNA. Although working models of miRNA-mediated gene silencing are successfully established using miRNA transfections and knockouts, the regulatory interaction between miRNA and long non-coding RNA (lncRNA) remain unknown. In particular, how the mRNA-resembling lncRNAs with 5' cap, 3' poly(A)-tail, or coding features, are regulated by miRNA is yet to be examined. We therefore investigated the functional interaction between miRNAs and lncRNAs with/without those features, in miRNA-transfected early zebrafish embryos. We observed that the greatest determinants of the miRNA-mediated silencing of lncRNAs were the 5' cap and 3' poly(A)-tails in lncRNAs, at both the post-transcriptional and translational levels. The lncRNAs confirmed to contain 5' cap, 3' poly(A)-tail, and the canonical miRNA target sites, were observed to be repressed in the level of both RNA and ribosome-protected fragment, while those with the miRNA target sites and without 5' cap and 3' poly(A)-tail, were not robustly repressed by miRNA introduction, thus suggesting a role as a miRNA-decoy. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY | - |
dc.title | Post-transcriptional and translational regulation of mRNA-like long non-coding RNAs by microRNAs in early developmental stages of zebrafish embryos | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Nam, Jin-Wu | - |
dc.identifier.doi | 10.5483/BMBRep.2017.50.4.025 | - |
dc.identifier.scopusid | 2-s2.0-85019001023 | - |
dc.identifier.wosid | 000400527600013 | - |
dc.identifier.bibliographicCitation | BMB REPORTS, v.50, no.4, pp.226 - 231 | - |
dc.relation.isPartOf | BMB REPORTS | - |
dc.citation.title | BMB REPORTS | - |
dc.citation.volume | 50 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 226 | - |
dc.citation.endPage | 231 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002218057 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | OPEN READING FRAMES | - |
dc.subject.keywordPlus | COMPETING ENDOGENOUS RNA | - |
dc.subject.keywordPlus | MUSCLE DIFFERENTIATION | - |
dc.subject.keywordPlus | MAMMALIAN MICRORNAS | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | EVOLUTION | - |
dc.subject.keywordPlus | STABILITY | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | COMPLEMENTARITY | - |
dc.subject.keywordPlus | DEGRADATION | - |
dc.subject.keywordAuthor | lncRNA | - |
dc.subject.keywordAuthor | miRNA | - |
dc.subject.keywordAuthor | sORF | - |
dc.subject.keywordAuthor | 3 &apos | - |
dc.subject.keywordAuthor | poly(A)-tail | - |
dc.subject.keywordAuthor | 5 &apos | - |
dc.subject.keywordAuthor | cap | - |
dc.identifier.url | http://koreascience.or.kr/article/JAKO201716556450924.page | - |
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