Construction of 3-D Cellular Multi-Layers with Extracellular Matrix Assembly Using Magnetic Nanoparticles
DC Field | Value | Language |
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dc.contributor.author | Lee, Yu Bin | - |
dc.contributor.author | Lee, Joong-Yup | - |
dc.contributor.author | Ahmad, Taufiq | - |
dc.contributor.author | Bak, Seongwoo | - |
dc.contributor.author | Lee, Jinkyu | - |
dc.contributor.author | Kim, Hwan D. | - |
dc.contributor.author | Park, Tai Hyun | - |
dc.contributor.author | Hwang, Nathaniel S. | - |
dc.contributor.author | Lim, Youn Mook | - |
dc.contributor.author | Shin, Heungsoo | - |
dc.date.accessioned | 2021-08-02T16:26:54Z | - |
dc.date.available | 2021-08-02T16:26:54Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2016-10 | - |
dc.identifier.issn | 1550-7033 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/22130 | - |
dc.description.abstract | Construction of 3-dimensional (3-D) engineered tissue is increasingly being investigated for use in drug discovery and regenerative medicine. Here, we developed multi-layered 3-D cellular assembly by using magnetic nanoparticles (MNP) isolated from Magnetospirillum sp. AMB-1 magnetotactic bacteria. Magnetized human dermal fibroblasts (HDFBs) were prepared by treatment with the MNP, induced to form 3-D assembly under a magnetic field. Analyses including LIVE/DEAD assay, transmission electron microscopy revealed that the MNP were internalized via clathrin-mediated endocytosis without cytotoxicity. The magnetized HDFBs could build 3-D structure as a function of seeding density. When the highest seeding density (5 x 10(5) cells/mm(2)) was used, the thickness of assembly was 41.0 +/- 1.69 mu m, with approximately 9.3 +/- 1.6 cell layers being formed. Immunofluorescence staining confirmed homogeneous distribution of ECM and junction proteins throughout the 3-D assembly. Real-time PCR analysis showed decrease in expression levels of collagen types I and IV but increase in that of connexin 43 in the 3-D assembly compared with the 2-D culture. Finally, we demonstrated that the discernible layers can be formed hierarchically by serial assembly. In conclusion, our study showed that a multi-layered structure can be easily prepared using magnetically-assisted cellular assembly with highlighting cell-cell and cell-ECM communication. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.title | Construction of 3-D Cellular Multi-Layers with Extracellular Matrix Assembly Using Magnetic Nanoparticles | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Heungsoo | - |
dc.identifier.doi | 10.1166/jbn.2016.2303 | - |
dc.identifier.scopusid | 2-s2.0-84990841912 | - |
dc.identifier.wosid | 000386486800007 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v.12, no.10, pp.1916 - 1928 | - |
dc.relation.isPartOf | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | - |
dc.citation.volume | 12 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1916 | - |
dc.citation.endPage | 1928 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | COCULTURE MODEL | - |
dc.subject.keywordPlus | SHEETS | - |
dc.subject.keywordPlus | FABRICATION | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | ENDOCYTOSIS | - |
dc.subject.keywordPlus | GENERATION | - |
dc.subject.keywordPlus | SPHEROIDS | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | TISSUES | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordAuthor | Hierarchical Tissue Mimicry | - |
dc.subject.keywordAuthor | Multi-Layered Structure | - |
dc.subject.keywordAuthor | 3-D Cellular Assembly | - |
dc.subject.keywordAuthor | Magnetic Nanoparticles | - |
dc.subject.keywordAuthor | Cellular Internalization | - |
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