Coxsackievirus and Adenovirus Receptor, a Tight Junction Protein, in Peri-Implantation Mouse Embryos
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Yeong Seok | - |
dc.contributor.author | Nah, Won Heum | - |
dc.contributor.author | Choi, Bomi | - |
dc.contributor.author | Kim, Seok Hyun | - |
dc.contributor.author | Gye, Myung Chan | - |
dc.date.accessioned | 2021-08-02T16:29:53Z | - |
dc.date.available | 2021-08-02T16:29:53Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2016-07 | - |
dc.identifier.issn | 0006-3363 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/22325 | - |
dc.description.abstract | To understand the role of Coxsackievirus and adenovirus receptor (CAR), a tight junction (TJ) protein, in peri-implantation embryos, developmental expression of CAR and its role in paracellular permeability were examined in mouse embryos. Splice variants for transmembrane CAR, Car1, Car2, and Car3 mRNA, were expressed from 2-cell, morula, and blastocyst stages onward, respectively, whereas mRNA for soluble CAR was expressed in MII oocytes and 4-cell stage onward. On Western blot, ∼46 kDa CAR proteins were detected in blastocysts. During the 4-cell embryos to morula stage, CAR was gradually concentrated at the contacts between blastomeres. In blastocysts, CAR was expressed at the cell contacts within the inner cell mass as well as in the trophectoderm (TE) where CAR was found together with ZO1 at the apical contacts, suggesting that CAR builds up apical TJs in TE and mediates cell adhesion in TE and inner cell mass. In blastocysts, CAR-blocking antibodies under Ca2+ switching increased the dextran permeability and decreased the volume of blastocoel and H19 and Cdx2 mRNA, suggesting the pivotal role of CAR in the blastocyst development and paracellular permeability barrier in TE. CAR was expressed in TE of implanting embryos as well as endometrial epithelium, suggesting the involvement of CAR in the interaction between implanting embryos and endometrium. At 5–6 days postcoitum, CAR was expressed together with ZO1 in the primitive endoderm, visceral endoderm, and epiblasts facing the pro-amniotic cavity, suggesting that CAR TJs contribute to the separation of epiblast from the blastocoel and development of the pro-amniotic cavity within epiblasts. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | OXFORD UNIV PRESS INC | - |
dc.title | Coxsackievirus and Adenovirus Receptor, a Tight Junction Protein, in Peri-Implantation Mouse Embryos | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gye, Myung Chan | - |
dc.identifier.doi | 10.1095/biolreprod.115.138099 | - |
dc.identifier.scopusid | 2-s2.0-84982957511 | - |
dc.identifier.wosid | 000379573600005 | - |
dc.identifier.bibliographicCitation | BIOLOGY OF REPRODUCTION, v.95, no.1, pp.1 - 11 | - |
dc.relation.isPartOf | BIOLOGY OF REPRODUCTION | - |
dc.citation.title | BIOLOGY OF REPRODUCTION | - |
dc.citation.volume | 95 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 11 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Reproductive Biology | - |
dc.relation.journalWebOfScienceCategory | Reproductive Biology | - |
dc.subject.keywordPlus | HUMAN BLADDER-CANCER | - |
dc.subject.keywordPlus | GERM-CELLS | - |
dc.subject.keywordPlus | BLASTOCYST FORMATION | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | CAR | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | BIOGENESIS | - |
dc.subject.keywordPlus | TROPHECTODERM | - |
dc.subject.keywordPlus | IMPACT | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordAuthor | CAR | - |
dc.subject.keywordAuthor | mouse | - |
dc.subject.keywordAuthor | peri-implantation embryo | - |
dc.subject.keywordAuthor | tight junction | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.