Systemic Delivery of an Oncolytic Adenovirus Expressing Decorin for the Treatment of Breast Cancer Bone Metastases
DC Field | Value | Language |
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dc.contributor.author | Yang, Yuefeng | - |
dc.contributor.author | Xu, Weidong | - |
dc.contributor.author | Neill, Thomas | - |
dc.contributor.author | Hu, Zebin | - |
dc.contributor.author | Wang, Chi-Hsiung | - |
dc.contributor.author | Xiao, Xianghui | - |
dc.contributor.author | Stock, Stuart R. | - |
dc.contributor.author | Guise, Theresa | - |
dc.contributor.author | Yun, Chae-Ok | - |
dc.contributor.author | Brendler, Charles B. | - |
dc.contributor.author | Iozzo, Renato V. | - |
dc.contributor.author | Seth, Prem | - |
dc.date.accessioned | 2021-08-02T17:52:09Z | - |
dc.date.available | 2021-08-02T17:52:09Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2015-12 | - |
dc.identifier.issn | 1043-0342 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/24771 | - |
dc.description.abstract | The development of novel therapies for breast cancer bone metastasis is a major unmet medical need. Toward that end, we have constructed an oncolytic adenovirus, Ad.dcn, and a nonreplicating adenovirus, Ad(E1-).dcn, both containing the human decorin gene. Our in vitro studies showed that Ad.dcn produced high levels of viral replication and the decorin protein in the breast tumor cells. Ad(E1-).dcn-mediated decorin expression in MDA-MB-231 cells downregulated the expression of Met, -catenin, and vascular endothelial growth factor A, all of which are recognized decorin targets and play pivotal roles in the progression of breast tumor growth and metastasis. Adenoviral-mediated decorin expression inhibited cell migration and induced mitochondrial autophagy in MDA-MB-231 cells. Mice bearing MDA-MB-231-luc skeletal metastases were systemically administered with the viral vectors, and skeletal tumor growth was monitored over time. The results of bioluminescence imaging and X-ray radiography indicated that Ad.dcn and Ad(E1-).dcn significantly inhibited the progression of bone metastases. At the terminal time point, histomorphometric analysis, micro-computed tomography, and bone destruction biomarkers showed that Ad.dcn and Ad(E1-).dcn reduced tumor burden and inhibited bone destruction. A nonreplicating adenovirus Ad(E1-).luc expressing the luciferase 2 gene had no significant effect on inhibiting bone metastases, and in several assays, Ad.dcn and Ad(E1-).dcn were better than Ad.luc, a replicating virus expressing the luciferase 2 gene. Our data suggest that adenoviral replication coupled with decorin expression could produce effective antitumor responses in a MDA-MB-231 bone metastasis model of breast cancer. Thus, Ad.dcn could potentially be developed as a candidate gene therapy vector for treating breast cancer bone metastases. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MARY ANN LIEBERT, INC | - |
dc.title | Systemic Delivery of an Oncolytic Adenovirus Expressing Decorin for the Treatment of Breast Cancer Bone Metastases | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yun, Chae-Ok | - |
dc.identifier.doi | 10.1089/hum.2015.098 | - |
dc.identifier.scopusid | 2-s2.0-84949886842 | - |
dc.identifier.wosid | 000366833500006 | - |
dc.identifier.bibliographicCitation | HUMAN GENE THERAPY, v.26, no.12, pp.813 - 825 | - |
dc.relation.isPartOf | HUMAN GENE THERAPY | - |
dc.citation.title | HUMAN GENE THERAPY | - |
dc.citation.volume | 26 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 813 | - |
dc.citation.endPage | 825 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Genetics & Heredity | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Genetics & Heredity | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.subject.keywordPlus | GROWTH-FACTOR-BETA | - |
dc.subject.keywordPlus | TGF-BETA | - |
dc.subject.keywordPlus | MEDIATED INHIBITION | - |
dc.subject.keywordPlus | EXTRACELLULAR-MATRIX | - |
dc.subject.keywordPlus | CARCINOMA CELLS | - |
dc.subject.keywordPlus | AUTOPHAGY | - |
dc.subject.keywordPlus | MICROENVIRONMENT | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | MITOPHAGY | - |
dc.subject.keywordPlus | DYNAMICS | - |
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