Using a magnetic field to redirect an oncolytic adenovirus complexed with iron oxide augments gene therapy efficacy
DC Field | Value | Language |
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dc.contributor.author | Choi, Joung-Woo | - |
dc.contributor.author | Park, Ji Won | - |
dc.contributor.author | Na, Youjin | - |
dc.contributor.author | Jung, Soo-Jung | - |
dc.contributor.author | Hwang, June Kyu | - |
dc.contributor.author | Choi, Dongho | - |
dc.contributor.author | Lee, Kyeong Geun | - |
dc.contributor.author | Yun, Chae-Ok | - |
dc.date.accessioned | 2021-08-02T17:53:29Z | - |
dc.date.available | 2021-08-02T17:53:29Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/24839 | - |
dc.description.abstract | Adenovirus (Ad) is a widely used vector for cancer gene therapy but its therapeutic efficacy is limited by low coxsackievirus and adenovirus receptor (CAR) expression in tumors and non-specifically targeted infection. Ad infectivity and specificity can be markedly improved by creating Ad-magnetic nanoparticles cluster complexes and directing their migration with an external magnetic field (MGF). We electrostatically complexed GFP-expressing, replication-incompetent Ad (dAd) with PEGylated and cross-linked iron oxide nanoparticles (PCION), generating dAd-PCION complexes. The dAd-PCION showed increased transduction efficiency, independent of CAR expression, in the absence or presence of an MGF. Cancer cell killing and intracellular oncolytic Ad (HmT)-PCION replication significantly increased with MGF exposure. Site-directed, magnetically-targeted delivery of the HmT-PCION elicited significantly greater therapeutic efficacy versus treatment with naked HmT or HmT-PCION without MGF in CAR-negative MCF7 tumors. Immunohistochemical tumor analysis showed increased oncolytic Ad replication in tumors following infection by HmT-PCION using an MGF. Whole-body bioluminescence imaging of tumor-bearing mice showed a 450-fold increased tumor-to-liver ratio for HmT-PCION with, versus without, MGF. These results demonstrate the feasibility and potential of external MGF-responsive PCION-coated oncolytic Ads as smart hybrid vectors for cancer gene therapy. (C) 2015 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Using a magnetic field to redirect an oncolytic adenovirus complexed with iron oxide augments gene therapy efficacy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Choi, Dongho | - |
dc.contributor.affiliatedAuthor | Lee, Kyeong Geun | - |
dc.contributor.affiliatedAuthor | Yun, Chae-Ok | - |
dc.identifier.doi | 10.1016/j.biomaterials.2015.07.001 | - |
dc.identifier.scopusid | 2-s2.0-84937408013 | - |
dc.identifier.wosid | 000358806600018 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.65, pp.163 - 174 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 65 | - |
dc.citation.startPage | 163 | - |
dc.citation.endPage | 174 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | POLYETHYLENE-GLYCOL | - |
dc.subject.keywordPlus | MAGNETOFECTION | - |
dc.subject.keywordPlus | NANOPARTICLES | - |
dc.subject.keywordPlus | VECTORS | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordAuthor | Cancer gene therapy | - |
dc.subject.keywordAuthor | Oncolytic adenovirus | - |
dc.subject.keywordAuthor | Magnetofection | - |
dc.subject.keywordAuthor | PEGylated and cross-linked iron oxide nanoparticles (PCION) | - |
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