Pachymic Acid Induces Apoptosis of EJ Bladder Cancer Cells by DR5 Up-Regulation, ROS Generation, Modulation of Bcl-2 and IAP Family Members
DC Field | Value | Language |
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dc.contributor.author | Jeong, Jin-Woo | - |
dc.contributor.author | Lee, Won Sup | - |
dc.contributor.author | Go, Se-il | - |
dc.contributor.author | Nagappan, Arulkumar | - |
dc.contributor.author | Baek, Jun Young | - |
dc.contributor.author | Lee, Jae-Dong | - |
dc.contributor.author | Lee, Su-Jae | - |
dc.contributor.author | Park, Cheol | - |
dc.contributor.author | Kim, Gi Young | - |
dc.contributor.author | Kim, Hye Jung | - |
dc.contributor.author | Kim, Gon-Sup | - |
dc.contributor.author | Kwon, Taeg Kyu | - |
dc.contributor.author | Ryu, Chung Ho | - |
dc.contributor.author | Shin, Sung Chul | - |
dc.contributor.author | Choi, Yung Hyun | - |
dc.date.accessioned | 2021-08-02T17:53:31Z | - |
dc.date.available | 2021-08-02T17:53:31Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2015-10 | - |
dc.identifier.issn | 0951-418X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/24840 | - |
dc.description.abstract | Pachymic acid (PA) is a lanostane-type triterpenoid derived from Poria cocos mushroom that possess various biological effects such as anti-cancer, antiinflammatory and anti-metastasis effects. In this study, we investigated the anti-cancer effects of PA in EJ bladder cancer cells. The results showed that PA significantly inhibited proliferation of EJ cells in a dose-dependent manner. PA induced accumulation of sub-G1 DNA content (apoptotic cell population), apoptotic bodies and chromatin condensation and DNA fragmentation in EJ cells in a dose-dependent manner. PA also induces activation of caspase-3, -8 and -9, and subsequent cleavage of poly (ADP-ribose) polymerase, and significantly suppressed the inhibitor of apoptosis protein family proteins in a dose-dependent manner. Furthermore, PA activates Bid and induced the loss of mitochondrial membrane potential (Delta Psi(m)) with up-regulated pro-apoptotic proteins (Bax and Bad), down-regulated anti-apoptotic proteins (Bcl-2 and Bcl-xL) and cytochrome c release. In turn, PA increased the generation of reactive oxygen species (ROS); also, the ROS production was blocked by N-acetyl-L-cysteine. The expressions of TNF-related apoptosis inducing ligand and death receptor 5 were up-regulated by PA in a dose-dependent manner, suggesting extrinsic pathway also involved in PA-induced apoptosis. This study provides evidence that PA might be useful in the treatment of human bladder cancer. Copyright (c) 2015 John Wiley & Sons, Ltd. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Pachymic Acid Induces Apoptosis of EJ Bladder Cancer Cells by DR5 Up-Regulation, ROS Generation, Modulation of Bcl-2 and IAP Family Members | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Su-Jae | - |
dc.identifier.doi | 10.1002/ptr.5402 | - |
dc.identifier.scopusid | 2-s2.0-84943583006 | - |
dc.identifier.wosid | 000362792900008 | - |
dc.identifier.bibliographicCitation | PHYTOTHERAPY RESEARCH, v.29, no.10, pp.1516 - 1524 | - |
dc.relation.isPartOf | PHYTOTHERAPY RESEARCH | - |
dc.citation.title | PHYTOTHERAPY RESEARCH | - |
dc.citation.volume | 29 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 1516 | - |
dc.citation.endPage | 1524 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | OXYGEN SPECIES ROS | - |
dc.subject.keywordPlus | CYTOCHROME-C | - |
dc.subject.keywordPlus | QUERCETIN | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | FRUIT | - |
dc.subject.keywordPlus | RISK | - |
dc.subject.keywordAuthor | pachymic acid | - |
dc.subject.keywordAuthor | EJ bladder cancer cells | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | death receptor | - |
dc.subject.keywordAuthor | caspase | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
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