Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model
DC Field | Value | Language |
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dc.contributor.author | Lee, Won Jai | - |
dc.contributor.author | Lee, Ju Hee | - |
dc.contributor.author | Ahn, Hyo Min | - |
dc.contributor.author | Song, Seung Yong | - |
dc.contributor.author | Kim, Yong Oock | - |
dc.contributor.author | Lew, Dae Hyun | - |
dc.contributor.author | Yun, Chae-Ok | - |
dc.date.accessioned | 2021-08-02T17:54:27Z | - |
dc.date.available | 2021-08-02T17:54:27Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2015-09 | - |
dc.identifier.issn | 0032-1052 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/24877 | - |
dc.description.abstract | Background: The 90-kDa heat-shock protein (heat-shock protein 90) is an abundant cytosolic chaperone, and inhibition of heat-shock protein 90 by 17-allylamino-17-demethoxygeldanamycin (17-AAG) compromises transforming growth factor (TGF)--mediated transcriptional responses by enhancing TGF- receptor I and II degradation, thus preventing Smad2/3 activation. In this study, the authors evaluated whether heat-shock protein 90 regulates TGF- signaling in the pathogenesis and treatment of keloids. Methods: Keloid fibroblasts were treated with 17-AAG (10 M), and mRNA levels of collagen types I and III were determined by real-time reverse- transcriptase polymerase chain reaction. Also, secreted TGF-1 was assessed by enzyme-linked immunosorbent assay. The effect of 17-AAG on protein levels of Smad2/3 complex was determined by Western blot analysis. In addition, in 17-AAG-treated keloid spheroids, the collagen deposition and expression of major extracellular matrix proteins were investigated by means of Masson trichrome staining and immunohistochemistry. Results: The authors found that heat-shock protein 90 is overexpressed in human keloid tissue compared with adjacent normal tissue, and 17-AAG decreased mRNA levels of type I collagen, secreted TGF-ss 1, and Smad2/3 complex protein expression in keloid fibroblasts. Masson trichrome staining revealed that collagen deposition was decreased in 17-AAG-treated keloid spheroids, and immunohistochemical analysis showed that expression of collagen types I and III, elastin, and fibronectin was markedly decreased in 17-AAG-treated keloid spheroids. Conclusion: These results suggest that the antifibrotic action of heat-shock protein 90 inhibitors such as 17-AAG may have therapeutic effects on keloids. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | LIPPINCOTT WILLIAMS & WILKINS | - |
dc.title | Heat Shock Protein 90 Inhibitor Decreases Collagen Synthesis of Keloid Fibroblasts and Attenuates the Extracellular Matrix on the Keloid Spheroid Model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yun, Chae-Ok | - |
dc.identifier.doi | 10.1097/PRS.0000000000001538 | - |
dc.identifier.scopusid | 2-s2.0-84940978450 | - |
dc.identifier.wosid | 000360685100001 | - |
dc.identifier.bibliographicCitation | PLASTIC AND RECONSTRUCTIVE SURGERY, v.136, no.3, pp.328e - 337e | - |
dc.relation.isPartOf | PLASTIC AND RECONSTRUCTIVE SURGERY | - |
dc.citation.title | PLASTIC AND RECONSTRUCTIVE SURGERY | - |
dc.citation.volume | 136 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 328e | - |
dc.citation.endPage | 337e | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | HSP90 | - |
dc.subject.keywordPlus | PATHOGENESIS | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | SCAR | - |
dc.subject.keywordPlus | P38 | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | ACTIVATION | - |
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