Enhanced therapeutic efficacy of an adenovirus-PEI-bile-acid complex in tumors with low coxsackie and adenovirus receptor expression
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Cho-Hee | - |
dc.contributor.author | Kasala, Dayananda | - |
dc.contributor.author | Na, Youjin | - |
dc.contributor.author | Lee, Min Sang | - |
dc.contributor.author | Kim, Sung Wan | - |
dc.contributor.author | Jeong, Ji Hoon | - |
dc.contributor.author | Yun, Chae-Ok | - |
dc.date.accessioned | 2021-08-02T18:30:19Z | - |
dc.date.available | 2021-08-02T18:30:19Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2014-07 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/25830 | - |
dc.description.abstract | Adenovirus (Ad) is a potential vehicle for cancer gene therapy. However, cells that express low levels of the coxsackie and adenovirus receptor (CAR) demonstrate poor Ad infection efficiency. We developed a bile acid-conjugated poly(ethyleneimine) (DA3)-coated Ad complex (Ad/DA3) to enhance Ad transduction efficiency. The size distribution and zeta potential of Ad/DA3 increased to 324 +/- 3.08 nm and 10.13 +/- 0.21 my, respectively, compared with those of naked Ad (108 +/- 2.26 nm and -17.7 +/- 1.5 mV). The transduction efficiency of Ad/DA3 increased in a DA3 polymer concentration-dependent manner. Enhanced gene transfer by Ad/DA3 was more evident in CAR-moderate and CAR-negative cancer cells. Competition assays with a CAR-specific antibody revealed that internalization of Ad/DA3 was not mediated primarily by CAR but involved clathrin-, caveolae-, and macropinocytosis-mediated endocytosis. Cancer cell death was significantly increased when oncolytic Ad and DA3 were complexed (RdB-KOX/DA3) compared to that of naked oncolytic Ad and was inversely proportional to CAR levels. Importantly, RdB-KOX/DA3 significantly enhanced apoptosis, reduced angiogenesis, reduced proliferation, and increased active viral replication in human tumor xenografts compared to that of naked Ad. These results demonstrate that a hybrid vector system can increase the efficacy of oncolytic Ad viro-therapy, particularly in CAR-limited tumors. (C) 2014 Elsevier Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Enhanced therapeutic efficacy of an adenovirus-PEI-bile-acid complex in tumors with low coxsackie and adenovirus receptor expression | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yun, Chae-Ok | - |
dc.identifier.doi | 10.1016/j.biomaterials.2014.03.060 | - |
dc.identifier.scopusid | 2-s2.0-84899474705 | - |
dc.identifier.wosid | 000336346000009 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.35, no.21, pp.5505 - 5516 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 35 | - |
dc.citation.number | 21 | - |
dc.citation.startPage | 5505 | - |
dc.citation.endPage | 5516 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | ONCOLYTIC ADENOVIRUS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | POLYETHYLENIMINE | - |
dc.subject.keywordPlus | TRANSDUCTION | - |
dc.subject.keywordPlus | REPLICATION | - |
dc.subject.keywordPlus | EFFICIENCY | - |
dc.subject.keywordPlus | VECTOR | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | Oncolytic adenovirus | - |
dc.subject.keywordAuthor | Cancer gene therapy | - |
dc.subject.keywordAuthor | Deoxycholic acid | - |
dc.subject.keywordAuthor | Poly(ethyleneimine) | - |
dc.subject.keywordAuthor | Coxsackie and adenovirus receptor | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.