A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells
DC Field | Value | Language |
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dc.contributor.author | Kim, A. D. | - |
dc.contributor.author | Kang, K. A. | - |
dc.contributor.author | Kim, H. S. | - |
dc.contributor.author | Kim, D. H. | - |
dc.contributor.author | Choi, Y. H. | - |
dc.contributor.author | Lee, S. J. | - |
dc.contributor.author | Kim, H. S. | - |
dc.contributor.author | Hyun, J. W. | - |
dc.date.accessioned | 2021-08-02T18:55:07Z | - |
dc.date.available | 2021-08-02T18:55:07Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2013-08 | - |
dc.identifier.issn | 2041-4889 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/26672 | - |
dc.description.abstract | Compound K (20-O-(beta-D-glucopyranosyl)-20(S)-protopanaxadiol) is an active metabolite of ginsenosides and induces apoptosis in various types of cancer cells. This study investigated the role of autophagy in compound K-induced cell death of human HCT-116 colon cancer cells. Compound K activated an autophagy pathway characterized by the accumulation of vesicles, the increased positive acridine orange-stained cells, the accumulation of LC3-II, and the elevation of autophagic flux. Whereas blockade of compound K-induced autophagy by 3-methyladenein and bafilomycin A1 significantly increased cell viability. In addition, compound K augmented the time-dependent expression of the autophagy-related proteins Atg5, Atg6, and Atg7. However, knockdown of Atg5, Atg6, and Atg7 markedly inhibited the detrimental impact of compound K on LC3-II accumulation and cell vitality. Compound K-provoked autophagy was also linked to the generation of intracellular reactive oxygen species (ROS); both of these processes were mitigated by the pre-treatment of cells with the antioxidant N-acetylcysteine. Moreover, compound K activated the c-Jun NH2-terminal kinase (JNK) signaling pathway, whereas downregulation of JNK by its specific inhibitor SP600125 or by small interfering RNA against JNK attenuated autophagy-mediated cell death in response to compound K. Compound K also provoked apoptosis, as evidenced by an increased number of apoptotic bodies and sub-G(1) hypodiploid cells, enhanced activation of caspase-3 and caspase-9, and modulation of Bcl-2 and Bcl-2-associated X protein expression. Notably, compound K-stimulated autophagy as well as apoptosis was induced by disrupting the interaction between Atg6 and Bcl-2. Taken together, these results indicate that the induction of autophagy and apoptosis by compound K is mediated through ROS generation and JNK activation in human colon cancer cells. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | A ginseng metabolite, compound K, induces autophagy and apoptosis via generation of reactive oxygen species and activation of JNK in human colon cancer cells | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, S. J. | - |
dc.identifier.doi | 10.1038/cddis.2013.273 | - |
dc.identifier.scopusid | 2-s2.0-84882658193 | - |
dc.identifier.wosid | 000324146000007 | - |
dc.identifier.bibliographicCitation | CELL DEATH & DISEASE, v.4 | - |
dc.relation.isPartOf | CELL DEATH & DISEASE | - |
dc.citation.title | CELL DEATH & DISEASE | - |
dc.citation.volume | 4 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | DEATH | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordPlus | SAPONIN | - |
dc.subject.keywordPlus | LC3 | - |
dc.subject.keywordPlus | CROSSTALK | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | ASSAY | - |
dc.subject.keywordAuthor | apoptosis | - |
dc.subject.keywordAuthor | autophagy | - |
dc.subject.keywordAuthor | compound K | - |
dc.subject.keywordAuthor | reactive oxygen species | - |
dc.subject.keywordAuthor | colon cancer | - |
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