Relaxin-Expressing Adenovirus Decreases Collagen Synthesis and Up-Regulates Matrix Metalloproteinase Expression in Keloid Fibroblasts: In Vitro Experiments
DC Field | Value | Language |
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dc.contributor.author | Lee, Won Jai | - |
dc.contributor.author | Choi, Il-Kyu | - |
dc.contributor.author | Lee, Ju Hee | - |
dc.contributor.author | Lee, Jung-Sun | - |
dc.contributor.author | Kim, Yong Oock | - |
dc.contributor.author | Rah, Dong Kyun | - |
dc.contributor.author | Yun, Chae-Ok | - |
dc.date.accessioned | 2021-08-02T19:27:41Z | - |
dc.date.available | 2021-08-02T19:27:41Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2012-09 | - |
dc.identifier.issn | 0032-1052 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/27477 | - |
dc.description.abstract | Background: The hormone relaxin has been shown to affect the extracellular matrix by inhibiting collagen synthesis and expression in fibroblasts stimulated with a profibrotic agent. It also increases matrix metalloproteinase (MMP) expression. To investigate its effect on expression of collagen and MMPs in keloid fibroblasts and human dermal fibroblasts, the authors introduced a relaxin-expressing adenovirus (dE1-RGD/lacZ/RLX) into a human dermal fibroblast cell line and keloid fibroblasts. Methods: Both fibroblasts were infected with dE1-RGD/lacZ/RLX or control virus, and protein levels of relaxin and secreted transforming growth factor (TGF)-beta 1 were assessed by enzyme-linked immunosorbent assay, and mRNA levels of collagen type I, collagen type III, MMP-1, and MMP-3 were assessed by real-time reverse-transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay. Expression of Smad3 and phosphorylated Smad3 was also examined, and relaxin's effect on Smad2/3 complex localization was evaluated. Results: When human dermal fibroblasts and keloid fibroblasts were transduced with dE1-RGD/lacZ/RLX or dE1-RGD/lacZ (control), mRNA expression of type I and type III collagen was markedly decreased by relaxin regardless of TGF-beta (10 ng/ml) treatment. Expression of Smad3 and phosphorylated Smad3 was reduced in keloid fibroblasts and decreased translocation of Smad 2/3 complex from cytosols to the nucleus of the human dermal fibroblasts with TGF-beta after dE1-RGD/lacZ/RLX transduction, suggesting that relaxin reduces collagen synthesis by blocking TGF-beta signaling. Analyses revealed that MMP-1 and MMP-3 expression were significantly increased in human dermal fibroblasts and keloid fibroblasts after dE1-RGD/lacZ/RLX transduction. Conclusion: These results suggest that the antifibrotic effect of relaxin-expressing adenovirus may have therapeutic effects on keloids. (Plast. Reconstr. Surg. 130: 407e, 2012.) | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | LIPPINCOTT WILLIAMS & WILKINS | - |
dc.title | Relaxin-Expressing Adenovirus Decreases Collagen Synthesis and Up-Regulates Matrix Metalloproteinase Expression in Keloid Fibroblasts: In Vitro Experiments | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yun, Chae-Ok | - |
dc.identifier.doi | 10.1097/PRS.0b013e31825dbf56 | - |
dc.identifier.scopusid | 2-s2.0-84866029784 | - |
dc.identifier.wosid | 000308458300003 | - |
dc.identifier.bibliographicCitation | PLASTIC AND RECONSTRUCTIVE SURGERY, v.130, no.3, pp.407e - 417e | - |
dc.relation.isPartOf | PLASTIC AND RECONSTRUCTIVE SURGERY | - |
dc.citation.title | PLASTIC AND RECONSTRUCTIVE SURGERY | - |
dc.citation.volume | 130 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 407e | - |
dc.citation.endPage | 417e | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | GENE-THERAPY | - |
dc.subject.keywordPlus | HYPERTROPHIC SCARS | - |
dc.subject.keywordPlus | FIBROSIS | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | SECRETION | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | HORMONE | - |
dc.subject.keywordPlus | DEATH | - |
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