Combination of Radiotherapy and Adenovirus-Mediated p53 Gene Therapy for MDM2-Overexpressing Hepatocellular Carcinoma
DC Field | Value | Language |
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dc.contributor.author | Koom, WS | - |
dc.contributor.author | Park, SY | - |
dc.contributor.author | Kim, W | - |
dc.contributor.author | Kim, M | - |
dc.contributor.author | Kim, JS | - |
dc.contributor.author | Kim, H | - |
dc.contributor.author | Choi, IK | - |
dc.contributor.author | Yun, CO | - |
dc.contributor.author | Seong, J | - |
dc.date.accessioned | 2021-08-02T19:29:51Z | - |
dc.date.available | 2021-08-02T19:29:51Z | - |
dc.date.created | 2021-05-13 | - |
dc.date.issued | 2012-03 | - |
dc.identifier.issn | 0449-3060 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/27569 | - |
dc.description.abstract | The p53 gene plays a determinant role in radiation-induced cell death and its protein product is negatively regulated by MDM2. We investigated whether adenovirus-mediated modified p53 gene transfer, which blocks p53-MDM2 binding, is effective for radiation-induced cell death in hepatocellular carcinoma (HCC) at different MDM2 cellular levels. Human hepatocellular carcinoma cell lines expressing MDM2 at low levels (Huh7) and high levels (SK-Hep1) were used. Ad-p53 and Ad-p53vp are replication-deficient adenoviral vectors containing human wild-type or modified p53, respectively. The anti-tumor effect was highest for Ad-p53 + radiotherapy (RT) in the low-level MDM2 cells, whereas this effect was highest for Ad-p53vp + RT in the MDM2-overexpressing cells. In Huh-7 cells, Ad-p53 + RT decreased cell viability (32%) in vitro and inhibited tumor growth (enhancement factor, 1.86) in vivo. Additionally, p21 expression and apoptosis were increased. In contrast, in SK-Hep1 cells, Ad-p53vp + RT showed decreased cell viability (51%) in vitro and inhibition of tumor growth (enhancement factor, 3.07) in vivo. Caspase-3 expression and apoptosis were also increased. Adenovirus-expressing modified p53, which blocks p53-MDM2 binding, was effective in killing tumor cells overexpressing MDM2. Furthermore, the combination strategy for disruption of the p53-MDM2 interaction with RT demonstrated enhanced anti-tumor effects both in vitro and in vivo. | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.title | Combination of Radiotherapy and Adenovirus-Mediated p53 Gene Therapy for MDM2-Overexpressing Hepatocellular Carcinoma | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yun, CO | - |
dc.identifier.doi | 10.1269/jrr.11110 | - |
dc.identifier.scopusid | 2-s2.0-84863452345 | - |
dc.identifier.wosid | 000307196400006 | - |
dc.identifier.bibliographicCitation | JOURNAL OF RADIATION RESEARCH, v.53, no.2, pp.202 - 210 | - |
dc.relation.isPartOf | JOURNAL OF RADIATION RESEARCH | - |
dc.citation.title | JOURNAL OF RADIATION RESEARCH | - |
dc.citation.volume | 53 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 202 | - |
dc.citation.endPage | 210 | - |
dc.type.rims | ART | - |
dc.type.docType | 정기학술지(Article(Perspective Article포함)) | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Life Sciences & Biomedicine - Other Topics | - |
dc.relation.journalResearchArea | Oncology | - |
dc.relation.journalResearchArea | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.relation.journalWebOfScienceCategory | Biology | - |
dc.relation.journalWebOfScienceCategory | Oncology | - |
dc.relation.journalWebOfScienceCategory | Radiology, Nuclear Medicine & Medical Imaging | - |
dc.subject.keywordPlus | CANCER-THERAPY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | IONIZING-RADIATION | - |
dc.subject.keywordPlus | MDM2 | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | RADIOSENSITIZATION | - |
dc.subject.keywordPlus | TRANSACTIVATION | - |
dc.subject.keywordPlus | OVEREXPRESSION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MUTATIONS | - |
dc.subject.keywordAuthor | p53 | - |
dc.subject.keywordAuthor | MDM2 | - |
dc.subject.keywordAuthor | Radiotherapy | - |
dc.subject.keywordAuthor | Adenovirus | - |
dc.subject.keywordAuthor | Gene therapy | - |
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