Nicotinamide Phosphoribosyltransferase Is Essential for Interleukin-1 beta-mediated Dedifferentiation of Articular Chondrocytes via SIRT1 and Extracellular Signal-regulated Kinase (ERK) Complex Signaling
DC Field | Value | Language |
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dc.contributor.author | Hong, Eun-Hee | - |
dc.contributor.author | Yun, Hong Shik | - |
dc.contributor.author | Kim, Jongdoo | - |
dc.contributor.author | Um, Hong-Duck | - |
dc.contributor.author | Lee, Kee-Ho | - |
dc.contributor.author | Kang, Chang-Mo | - |
dc.contributor.author | Lee, Su-Jae | - |
dc.contributor.author | Chun, Jang-Soo | - |
dc.contributor.author | Hwang, Sang-Gu | - |
dc.date.accessioned | 2021-08-02T19:51:11Z | - |
dc.date.available | 2021-08-02T19:51:11Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2011-08 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/28094 | - |
dc.description.abstract | Although much is known about interleukin (IL)-1 beta and its role as a key mediator of cartilage destruction in osteoarthritis, only limited information is available on IL-1 beta signaling in chondrocyte dedifferentiation. Here, we have characterized the molecular mechanisms leading to the dedifferentiation of primary cultured articular chondrocytes by IL-1 beta treatment. IL-1 beta or lipopolysaccharide, but not phorbol 12-myristate 13-acetate, retinoic acid, or epidermal growth factor, induced nicotinamide phosphoribosyltransferase (NAMPT) expression, showing the association of inflammatory cytokines with NAMPT regulation. SIRT1, in turn, was activated NAMPT-dependently, without any alteration in the expression level. Activation or inhibition of SIRT1 oppositevely regulates IL-1 beta-mediated chondrocyte dedifferentiation, suggesting this protein as a key regulator of chondrocytes phenotype. SIRT1 activation promotes induction of ERK and p38 kinase activities, but not JNK, in response to IL-1 beta. Subsequently, ERK and p38 kinase activated by SIRT1 also induce SIRT1 activation, forming a positive feedback loop to sustain downstream signaling of these kinases. Moreover, we found that the SIRT1-ERK complex, but not SIRT1-p38, is engaged in IL-1 beta-induced chondrocyte dedifferentiation via a Sox-9-mediated mechanism. JNK is activated by IL-1 beta and modulates dedifferentiation of chondrocytes, but this pathway is independent on NAMPT-SIRT1 signaling. Based on these findings, we propose that IL-1 beta induces dedifferentiation of articular chondrocytes by up-regulation of SIRT1 activity enhanced by both NAMPT and ERK signaling. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | Nicotinamide Phosphoribosyltransferase Is Essential for Interleukin-1 beta-mediated Dedifferentiation of Articular Chondrocytes via SIRT1 and Extracellular Signal-regulated Kinase (ERK) Complex Signaling | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Su-Jae | - |
dc.identifier.doi | 10.1074/jbc.M111.219832 | - |
dc.identifier.scopusid | 2-s2.0-80051536994 | - |
dc.identifier.wosid | 000293557800076 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.286, no.32, pp.28619 - 28631 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 286 | - |
dc.citation.number | 32 | - |
dc.citation.startPage | 28619 | - |
dc.citation.endPage | 28631 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.subject.keywordPlus | GENE-EXPRESSION | - |
dc.subject.keywordPlus | DIFFERENTIATED PHENOTYPE | - |
dc.subject.keywordPlus | LIFE-SPAN | - |
dc.subject.keywordPlus | CARTILAGE | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | INFLAMMATION | - |
dc.subject.keywordPlus | RESTRICTION | - |
dc.subject.keywordPlus | EXTENSION | - |
dc.subject.keywordPlus | VISFATIN | - |
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