Thermally triggered self-assembly of kappa-casein amyloid nanofibrils and their nanomechanical properties
DC Field | Value | Language |
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dc.contributor.author | Lee, Jubong | - |
dc.contributor.author | Lee, Ji-Hye | - |
dc.contributor.author | Palk, Seung R. | - |
dc.contributor.author | Yeom, Bongjun | - |
dc.contributor.author | Char, Kookheon | - |
dc.date.accessioned | 2021-08-03T02:56:12Z | - |
dc.date.available | 2021-08-03T02:56:12Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2019-09 | - |
dc.identifier.issn | 0032-3861 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/32831 | - |
dc.description.abstract | Understanding the self-assembly process of amyloid nanofibrils is important in protein engineering for their potential uses in material sciences and medical applications. Here, we investigated the nanomechanical properties and structures of kappa-casein nanofibrils during thermally induced amyloidogenesis. After treatment with dithiothreitol to reduce disulfide bonds, kappa-casein from bovine milk was incubated at various temperatures from 37 to 95 degrees C. Twisted mature nanofibrils were only obtained at high thermal energies of 95 degrees C with fast growth kinetics. Nanomechanical studies using atomic force microscopy show the highest Young's moduli (2.6 +/- 0.4 GPa) for mature fibrils when compared to those of oligomers and protofibrils (0.6 +/- 0.1 and 2.2 +/- 0.3 GPa, respectively). These enhanced mechanical properties are mainly attributed to the twisted cores of the mature fibrils containing beta-sheet stacks. Amyloidogenesis of kappa-casein is highly dependent on the magnitude of thermal energy, determining both the types and shapes of the nanofibrils and their different mechanical properties. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Thermally triggered self-assembly of kappa-casein amyloid nanofibrils and their nanomechanical properties | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yeom, Bongjun | - |
dc.identifier.doi | 10.1016/j.polymer.2019.121626 | - |
dc.identifier.scopusid | 2-s2.0-85068362262 | - |
dc.identifier.wosid | 000487254600058 | - |
dc.identifier.bibliographicCitation | POLYMER, v.179, pp.1 - 10 | - |
dc.relation.isPartOf | POLYMER | - |
dc.citation.title | POLYMER | - |
dc.citation.volume | 179 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 10 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | THIOFLAVIN-T | - |
dc.subject.keywordPlus | MECHANICAL-PROPERTIES | - |
dc.subject.keywordPlus | SECONDARY NUCLEATION | - |
dc.subject.keywordPlus | CIRCULAR-DICHROISM | - |
dc.subject.keywordPlus | RAMAN-SPECTROSCOPY | - |
dc.subject.keywordPlus | FIBRIL FORMATION | - |
dc.subject.keywordPlus | MOLECULAR-BASIS | - |
dc.subject.keywordPlus | BOVINE CASEINS | - |
dc.subject.keywordPlus | BETA-CASEIN | - |
dc.subject.keywordPlus | AGGREGATION | - |
dc.subject.keywordAuthor | Amyloids | - |
dc.subject.keywordAuthor | Self-assembly | - |
dc.subject.keywordAuthor | Nanomechanical property | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S003238611930610X?via%3Dihub | - |
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