Effects of Homocysteine and Hyperglycemia on the Proliferation of Aortic Vascular Smooth Muscle Cells of Obese Type 2 Diabetes Rat
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yoo, Hyung Joon | - |
dc.contributor.author | Yu, Sung Hoon | - |
dc.contributor.author | Cho, Young Jung | - |
dc.contributor.author | Nam, Hong Woo | - |
dc.contributor.author | Kang, Dong Hyun | - |
dc.date.accessioned | 2021-07-30T05:12:13Z | - |
dc.date.available | 2021-07-30T05:12:13Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2017-06 | - |
dc.identifier.issn | 2508-4909 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/3533 | - |
dc.description.abstract | BACKGROUND The aim of this study was to investigate the role of homocysteine on the proliferation of rat aortic vascular smooth muscle cells (VSMCs) by measuring mitogen-activated protein (MAP) kinase under hyperglycemic conditions. METHODS Rat aortic VSMCs were isolated from Otsuka Long-Evans Tokushima Fatty and Long-Evans Tokushima Otsuka rats. VSMCs were incubated in the presence of homocysteine (1 mM) with/without PD98059 (30 µM) and wortmannin (300 nM) for 48 hours in different concentrations of glucose (5.5, 25 mM). Proliferation was evaluated by methylthiazoletetrazolium (MTT), fluorescence-activated cell sorting (FACS), and western blot analyses. RESULTS In MTT and FACS analyses, the proliferation of VSMCs was increased by homocysteine. After PD98059 (30 µM) and wortmannin (300 nM) treatment with homocysteine (1 mM), the increased proliferation of VSMC caused by homocysteine, decreased to control levels. In Western blot analysis, immunoexpression of phospho-p44/42 MAP kinase was significantly increased by homocysteine (1 mM). After PD98059 and wortmannin treatment, the increased immunoexpression of phospho-p44/42 MAP kinase was suppressed. CONCLUSION These results suggest that the MAP kinase and PI3 kinase pathways are the main mechanisms involved in rat VSMC proliferation caused by homocysteine under hyperglycemic conditions. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN GERIATRIC SOC | - |
dc.title | Effects of Homocysteine and Hyperglycemia on the Proliferation of Aortic Vascular Smooth Muscle Cells of Obese Type 2 Diabetes Rat | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Yu, Sung Hoon | - |
dc.identifier.doi | 10.4235/agmr.2017.21.2.78 | - |
dc.identifier.scopusid | 2-s2.0-85060048609 | - |
dc.identifier.bibliographicCitation | ANNALS OF GERIATRIC MEDICINE AND RESEARCH, v.21, no.2, pp.78 - 85 | - |
dc.relation.isPartOf | ANNALS OF GERIATRIC MEDICINE AND RESEARCH | - |
dc.citation.title | ANNALS OF GERIATRIC MEDICINE AND RESEARCH | - |
dc.citation.volume | 21 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 78 | - |
dc.citation.endPage | 85 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002236415 | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Geriatrics & Gerontology | - |
dc.relation.journalWebOfScienceCategory | Geriatrics & Gerontology | - |
dc.subject.keywordPlus | RISK-FACTOR | - |
dc.subject.keywordPlus | DISEASE | - |
dc.subject.keywordPlus | KINASE | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | HYPERHOMOCYSTEINEMIA | - |
dc.subject.keywordPlus | ATHEROSCLEROSIS | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | MIGRATION | - |
dc.subject.keywordPlus | PATHWAY | - |
dc.subject.keywordAuthor | Homocysteine | - |
dc.subject.keywordAuthor | Hyperglycemia | - |
dc.subject.keywordAuthor | Vascular smooth muscle cell | - |
dc.subject.keywordAuthor | Mitogen-activated protein kinase | - |
dc.subject.keywordAuthor | Phosphoinositide 3-kinase | - |
dc.identifier.url | https://www.e-agmr.org/journal/view.php?doi=10.4235/agmr.2017.21.2.78 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.