Human adipose-derived stem cell spheroids incorporating platelet-derived growth factor (PDGF) and bio-minerals for vascularized bone tissue engineering
DC Field | Value | Language |
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dc.contributor.author | Lee, Jinkyu | - |
dc.contributor.author | Lee, Sangmin | - |
dc.contributor.author | Ahmad, Taufiq | - |
dc.contributor.author | Perikamana, Sajeesh Kumar Madhurakkat | - |
dc.contributor.author | Lee, Jinki | - |
dc.contributor.author | Kim, Eun Mi | - |
dc.contributor.author | Shin, Heungsoo | - |
dc.date.accessioned | 2021-07-30T05:22:42Z | - |
dc.date.available | 2021-07-30T05:22:42Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2020-10 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4432 | - |
dc.description.abstract | Stem cells with mineralized materials have been used for bone regeneration; however, engineering the complex vascularized structure of the natural bone remains a challenge. Here, we developed platelet-derived growth factor (PDGF) and bio-mineral coated fibers which were then assembled with human adipose-derived stem cells (hADSCs) to form spheroids as building blocks for vascularized bone regeneration. The PDGF incorporated within the spheroid increased the proliferation of hADSCs, which was characterized by Ki-67 staining and DNA contents. Furthermore, the PDGF enhanced not only osteogenic differentiation, but also endothelial differentiation of hADSCs; the cells within the spheroids showed significantly greater gene expression by 2.46 +/- 0.14 fold for osteocalcin (OCN) and by 12.85 +/- 3.36 fold for von Willebrand factor (vWF) than those without PDGF. Finally, at two months following transplantation of PDGF-incorporated spheroids onto in vivo mouse calvarial defect, the regenerated bone area (42.48 +/- 10.84%) was significantly enhanced and the greatest number of capillaries and arterioles with indication of transplanted hADSCs were observed. Moreover, millimeter-scale in vitro tissue prepared by fused assembly of the spheroids exhibited greater mRNA expression-associated to endothelial lineage. Taken together, these findings indicate that stem cell spheroids incorporating PDGF and biominerals could be used as a module for successful vascularized bone regeneration. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Human adipose-derived stem cell spheroids incorporating platelet-derived growth factor (PDGF) and bio-minerals for vascularized bone tissue engineering | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Shin, Heungsoo | - |
dc.identifier.doi | 10.1016/j.biomaterials.2020.120192 | - |
dc.identifier.scopusid | 2-s2.0-85086381866 | - |
dc.identifier.wosid | 000555693800032 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.255, pp.1 - 16 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 255 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 16 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | OSTEOGENIC DIFFERENTIATION | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CRITICAL-SIZE | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | PROLIFERATION | - |
dc.subject.keywordPlus | BB | - |
dc.subject.keywordPlus | ENHANCEMENT | - |
dc.subject.keywordAuthor | Fragmented fibers | - |
dc.subject.keywordAuthor | Spheroid | - |
dc.subject.keywordAuthor | hADSCs | - |
dc.subject.keywordAuthor | Stem cell | - |
dc.subject.keywordAuthor | Angiogenic factor | - |
dc.subject.keywordAuthor | Bone regeneration | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0142961220304385?via%3Dihub | - |
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