Delivery of High Mobility Group Box-1 siRNA Using Brain-Targeting Exosomes for Ischemic Stroke Therapy
DC Field | Value | Language |
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dc.contributor.author | Kim, Min kyung | - |
dc.contributor.author | Kim, Gyeungyun | - |
dc.contributor.author | Hwang, Do Won | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2021-07-30T05:22:50Z | - |
dc.date.available | 2021-07-30T05:22:50Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2019-12 | - |
dc.identifier.issn | 1550-7033 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4480 | - |
dc.description.abstract | Ischemic strokes are caused by decreased blood flow into the brain, due to narrowed cerebral arteries. In the ischemic brain, high-mobility group box 1 (HMGB1) is released into extracellular spaces and induces inflammatory reactions. In this study, HMGB1 small interfering RNA (siRNA) was delivered into ischemic brains by intravenous administration using rabies virus glycoprotein (RVG) peptide-decorated exosomes. A fusion protein of RVG and Lamp2b was expressed in 293T cells. Since Lamp2b is an exosome membrane-integral protein, RVG-Lamp2b is integrated into the exosomes, producing RVG-decorated exosomes (RVG-Exo). HMGB1-siRNA was loaded into RVG-Exo and unmodified exosomes (Unmod-Exo) by electroporation. The exosomes were homogenous with a size of less than 50 nm and a negative surface charge. In vitro delivery assays showed that RVG-Exo showed higher efficiency to Neuro2A cells than Unmod-Exo. Also, HMGB1 levels were reduced more effectively by RVG-Exo/HMGB1-siRNA. In vivo delivery efficiency and therapeutic effects of RVG-Exo/HMGB1-siRNA were evaluated in a middle cerebral artery occlusion (MCAO) model. RVG-Exo/HMGB1-siRNA, Unmod-Exo/HMGB1-siRNA, and PEI25k/HMGB1-siRNA were administrated into the MCAO model intravenously through the tail vein. The results showed that HMGB1, tumor necrosis factor-alpha (TNF-alpha), and apoptosis levels in the brain were reduced in the RVG-Exo/HMGB1-siRNA group more efficiently than the other groups. In addition, the infarct size was decreased in the RVG-Exo/HMGB1 group more effectively than the other groups. These results suggest that RVG-Exo with HMGB1-siRNA may have potential as a therapeutic system for the treatment of ischemic strokes. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | AMER SCIENTIFIC PUBLISHERS | - |
dc.title | Delivery of High Mobility Group Box-1 siRNA Using Brain-Targeting Exosomes for Ischemic Stroke Therapy | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1166/jbn.2019.2866 | - |
dc.identifier.scopusid | 2-s2.0-85075813717 | - |
dc.identifier.wosid | 000498023900009 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v.15, no.12, pp.2401 - 2412 | - |
dc.relation.isPartOf | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF BIOMEDICAL NANOTECHNOLOGY | - |
dc.citation.volume | 15 | - |
dc.citation.number | 12 | - |
dc.citation.startPage | 2401 | - |
dc.citation.endPage | 2412 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 GENE | - |
dc.subject.keywordPlus | MEDIATED DELIVERY | - |
dc.subject.keywordPlus | PEPTIDE | - |
dc.subject.keywordPlus | HMGB1 | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | VESICLES | - |
dc.subject.keywordPlus | CURCUMIN | - |
dc.subject.keywordPlus | BARRIER | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | RAGE | - |
dc.subject.keywordAuthor | Exosome | - |
dc.subject.keywordAuthor | Rabies Virus Glycoprotein (RVG) Peptide | - |
dc.subject.keywordAuthor | High Mobility Group Box 1 (HMGB1) | - |
dc.subject.keywordAuthor | Small Interfering RNA (siRNA) | - |
dc.subject.keywordAuthor | Ischemic Stroke | - |
dc.identifier.url | https://www.ingentaconnect.com/content/asp/jbn/2019/00000015/00000012/art00009;jsessionid=1kwl42qqoo0j6.x-ic-live-03 | - |
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