Hydrogels with an embossed surface: An all-in-one platform for mass production and culture of human adipose-derived stem cell spheroids
DC Field | Value | Language |
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dc.contributor.author | Kim, Se-jeong | - |
dc.contributor.author | Park, Jaesung | - |
dc.contributor.author | Byun, Hayeon | - |
dc.contributor.author | Park, Young-Woo | - |
dc.contributor.author | Major, Luke G. | - |
dc.contributor.author | Lee, Dong Yun | - |
dc.contributor.author | Choi, Yu Suk | - |
dc.contributor.author | Shin, Heungsoo | - |
dc.date.accessioned | 2021-07-30T05:24:25Z | - |
dc.date.available | 2021-07-30T05:24:25Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2019-01 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4628 | - |
dc.description.abstract | Stem cell spheroids have been studied extensively in organoid culture and therapeutic transplantation. Herein, hydrogels with an embossed surface (HES) were developed as an all-in-one platform that can enable the rapid formation and culture of a large quantity of size-controllable stem cell spheroids. The embossed structure on the hydrogel was adjustable according to the grit designation of the sandpaper. Human adipose-derived stem cells (hADSCs) were rapidly assembled into spheroids on the hydrogel, with their size distribution precisely controlled from 95 +/- 6 pm to 181 +/- 15 mu m depending on surface roughness. The hADSC spheroids prepared from the HES demonstrated expression of sternness markers and differentiation capacity. In addition, HES-based spheroids showed significantly greater VEGF secretion than spheroids grown on a commercially available low-attachment culture plate. Exploiting those advantages, the HES-based spheroids were used for 3D bioprinting, and the spheroids within the 3D-printed construct showed improved retention and VEGF secretion compared to the same 3D structure containing single cell suspension. Collectively, HES would offer a useful platform for mass fabrication and culture of stem cell spheroids with controlled sizes for a variety of biomedical applications. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Hydrogels with an embossed surface: An all-in-one platform for mass production and culture of human adipose-derived stem cell spheroids | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Dong Yun | - |
dc.contributor.affiliatedAuthor | Shin, Heungsoo | - |
dc.identifier.doi | 10.1016/j.biomaterials.2018.10.025 | - |
dc.identifier.scopusid | 2-s2.0-85055993246 | - |
dc.identifier.wosid | 000450378200017 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, v.188, pp.198 - 212 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.citation.title | BIOMATERIALS | - |
dc.citation.volume | 188 | - |
dc.citation.startPage | 198 | - |
dc.citation.endPage | 212 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Engineering | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Engineering, Biomedical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | BONE-MARROW | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | MICROSTRUCTURE | - |
dc.subject.keywordPlus | VIABILITY | - |
dc.subject.keywordPlus | ROUGHNESS | - |
dc.subject.keywordPlus | CARTILAGE | - |
dc.subject.keywordPlus | EXPANSION | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | CHITOSAN | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordAuthor | 3D cell culture | - |
dc.subject.keywordAuthor | Stem cell spheroid | - |
dc.subject.keywordAuthor | 3D printing | - |
dc.subject.keywordAuthor | Surface structure | - |
dc.subject.keywordAuthor | Hydrogel | - |
dc.identifier.url | https://linkinghub.elsevier.com/retrieve/pii/S0142961218307397 | - |
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