Hyaluronate-alginate hybrid hydrogels modified with biomimetic peptides for controlling the chondrocyte phenotype
DC Field | Value | Language |
---|---|---|
dc.contributor.author | An, Hyoseok | - |
dc.contributor.author | Lee, Jae Won | - |
dc.contributor.author | Lee, Hyun Ji | - |
dc.contributor.author | Seo, Yerang | - |
dc.contributor.author | Park, Honghyun | - |
dc.contributor.author | Lee, Kuen Yong | - |
dc.date.accessioned | 2021-07-30T05:24:33Z | - |
dc.date.available | 2021-07-30T05:24:33Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-10 | - |
dc.identifier.issn | 0144-8617 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4669 | - |
dc.description.abstract | Hyaluronate-based hydrogels have been widely exploited as synthetic extracellular matrices in many tissue engineering applications, including cartilage tissue engineering. Hyaluronate-based hydrogels are typically prepared by chemical cross-linking reactions, in which chemical reagents may induce side effects, unless they are completely removed after the cross-linking reaction. We thus suggest the utilization of hybrid materials composed of hyaluronate as a main chain and alginate for physical cross-linking to simply form hydrogels in the presence of calcium ions under physiological conditions. In this study, we hypothesized that the introduction of biomimetic peptides to hyaluronate-alginate hybrid (HAH) hydrogels could be useful to regulate the chondrocyte phenotype, including chondrogenic differentiation. HAH was modified with the arginine-glycine-aspartate (RGD) peptide as a cell-matrix interaction motif and/or histidine-alanine-valine (HAV) as a cell-cell interaction motif. The HAV peptide is known to bind to cadherin, which is a key factor involved in homophilic cell-cell interactions as well as chondrogenesis. The viability and growth of mouse chondrocytes (ATDC5 cells) increased significantly when cultured on RGD-modified HAH hydrogels. Cell aggregates formed on HAV-modified HAH hydrogels, resulting in enhanced chondrogenic differentiation via enhanced cell-cell interactions by HAV modification. Interestingly, a synergistic effect of HAV and RGD peptides within HAH hydrogels on chondrogenesis was found in 3-D experiments in vitro. This approach to utilizing physically cross-linkable hyaluronate-based hydrogels presenting biomimetic peptides has potential applications in tissue engineering, including cartilage regeneration. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Hyaluronate-alginate hybrid hydrogels modified with biomimetic peptides for controlling the chondrocyte phenotype | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Kuen Yong | - |
dc.identifier.doi | 10.1016/j.carbpol.2018.06.016 | - |
dc.identifier.scopusid | 2-s2.0-85048555123 | - |
dc.identifier.wosid | 000438466500046 | - |
dc.identifier.bibliographicCitation | CARBOHYDRATE POLYMERS, v.197, pp.422 - 430 | - |
dc.relation.isPartOf | CARBOHYDRATE POLYMERS | - |
dc.citation.title | CARBOHYDRATE POLYMERS | - |
dc.citation.volume | 197 | - |
dc.citation.startPage | 422 | - |
dc.citation.endPage | 430 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Applied | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Organic | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | IN-VITRO CHONDROGENESIS | - |
dc.subject.keywordPlus | DRUG-DELIVERY | - |
dc.subject.keywordPlus | TISSUE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | DERIVATIVES | - |
dc.subject.keywordPlus | SCAFFOLDS | - |
dc.subject.keywordPlus | CATENIN | - |
dc.subject.keywordAuthor | Hyaluronate | - |
dc.subject.keywordAuthor | Alginate | - |
dc.subject.keywordAuthor | Arginine-glycine-aspartate | - |
dc.subject.keywordAuthor | Histidine-alanine-valine | - |
dc.subject.keywordAuthor | Chondrocyte phenotype | - |
dc.identifier.url | https://linkinghub.elsevier.com/retrieve/pii/S0144861718306702 | - |
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