Self-assembled polymeric micelles for combined delivery of anti-inflammatory gene and drug to the lungs by inhalation
DC Field | Value | Language |
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dc.contributor.author | Kim, Gyeungyun | - |
dc.contributor.author | Piao, Chunxian | - |
dc.contributor.author | Oh, Jungju | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2021-07-30T05:24:40Z | - |
dc.date.available | 2021-07-30T05:24:40Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-05 | - |
dc.identifier.issn | 2040-3364 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4706 | - |
dc.description.abstract | Acute lung injury (ALI) is a lung inflammatory disease for which pulmonary delivery of drug and gene could be a useful strategy. In this study, cholesterol-conjugated polyamidoamine (PAM-Chol) was synthesized and characterized as a carrier for combined delivery of anti-inflammatory gene and drug into the lungs by inhalation. The PAM-Chol formed self-assembled micelles in an aqueous solution with a critical micelle concentration of 0.22 mg ml(-1). An in vitro transfection assay to L2 lung epithelial cells showed that the PAM-Chol micelle had higher transfection efficiency than lipofectamine and polyethylenimine (25 kDa, PEI25k). As the anti-inflammatory drug, resveratrol was loaded into the cores of the PAM-Chol micelles using the oil-in-water emulsion/solvent evaporation method. In lipopolysaccharide (LPS)-activated macrophage cells, resveratrol-loaded PAM-Chol (PAM-Chol/Res) reduced pro-inflammatory cytokines, confirming the anti-inflammatory effects of resveratrol. In in vitro transfection assays to L2 cells, the PAM-Chol/Res micelles had transfection efficiency similar to that of PAM-Chol. The delivery of resveratrol or the heme oxygenase-1 gene (pHO-1) by inhalation was evaluated in an ALI animal model. Resveratrol delivery using the PAM-Chol/Res micelles inhibited the nuclear translocation of nuclear factor-kappa B (NF-kappa B) and reduced pro-inflammatory cytokines in the lungs. pHO-1 delivery using PAM-Chol induced HO-1 expression and reduced pro-inflammatory cytokines. However, the highest anti-inflammatory effects were obtained with combined delivery of pHO-1 and resveratrol using the pHO-1/PAM-Chol/Res complex, as demonstrated in cytokine assays and immunohistochemical studies. Therefore, the PAM-Chol micelle is an efficient carrier of resveratrol and pHO-1 into the lungs and could be useful for the treatment of ALI by inhalation. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Self-assembled polymeric micelles for combined delivery of anti-inflammatory gene and drug to the lungs by inhalation | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1039/c8nr00427g | - |
dc.identifier.scopusid | 2-s2.0-85046893383 | - |
dc.identifier.wosid | 000432096400019 | - |
dc.identifier.bibliographicCitation | NANOSCALE, v.10, no.18, pp.8503 - 8514 | - |
dc.relation.isPartOf | NANOSCALE | - |
dc.citation.title | NANOSCALE | - |
dc.citation.volume | 10 | - |
dc.citation.number | 18 | - |
dc.citation.startPage | 8503 | - |
dc.citation.endPage | 8514 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Physics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Physics, Applied | - |
dc.subject.keywordPlus | RESPIRATORY-DISTRESS-SYNDROME | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 GENE | - |
dc.subject.keywordPlus | POLYAMIDOAMINE DENDRIMERS | - |
dc.subject.keywordPlus | INFLAMMATORY MEDIATORS | - |
dc.subject.keywordPlus | ISCHEMIC BRAIN | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | INJURY | - |
dc.subject.keywordPlus | RESVERATROL | - |
dc.subject.keywordPlus | DEXAMETHASONE | - |
dc.subject.keywordPlus | THERAPY | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2018/NR/C8NR00427G | - |
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