Production and application of HMGB1 derived recombinant RAGE-antagonist peptide for anti-inflammatory therapy in acute lung injury
DC Field | Value | Language |
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dc.contributor.author | Lee, Seonyeong | - |
dc.contributor.author | Piao, Chunxian | - |
dc.contributor.author | Kim, Gyeungyun | - |
dc.contributor.author | Kim, Ji Yeon | - |
dc.contributor.author | Choi, Eunji | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2021-07-30T05:24:43Z | - |
dc.date.available | 2021-07-30T05:24:43Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-03 | - |
dc.identifier.issn | 0928-0987 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4725 | - |
dc.description.abstract | Acute lung injury (ALI) is an inflammatory lung disease caused by sepsis, infection, or ischemia-reperfusion. The receptor for advanced glycation end-products (RAGE) signaling pathway plays an important role in ALI. In this study, a novel RAGE-antagonist peptide (RAP) was produced as an inhibitor of the RAGE signaling pathway based on the RAGE-binding domain of high mobility group box-1 (HMGB1). Recombinant RAP was over-expressed and purified using nickel-affinity chromatography. In lipopolysaccharide-or HMGB1-activated RAW264.7 macrophage cells, RAP reduced the levels of pro-inflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6). RAP decreased the levels of cell surface RAGE and inhibited the nuclear translocation of nuclear factor-kappa B (NF-kappa B). These results imply that RAP decreases RAGE-mediated NF-kappa B activation and subsequent inflammatory reaction. For in vivo evaluation, RAP was delivered to the lungs of ALI-model animals via intratracheal administration. As a result, RAGE was down-regulated in the lung tissues by pulmonary delivery of RAP. Consequently, TNF-alpha, IL-6, and IL-1 beta were also reduced in broncoalveolar lavage fluid and the lung tissues of RAP-treated animals. Hematoxylin and eosin staining indicated that inflammation was decreased in RAP-treated animals. Collectively, these results suggest that RAP may be a useful treatment for ALI. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.title | Production and application of HMGB1 derived recombinant RAGE-antagonist peptide for anti-inflammatory therapy in acute lung injury | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1016/j.ejps.2017.12.019 | - |
dc.identifier.scopusid | 2-s2.0-85039845440 | - |
dc.identifier.wosid | 000424977500028 | - |
dc.identifier.bibliographicCitation | EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, v.114, pp.275 - 284 | - |
dc.relation.isPartOf | EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | - |
dc.citation.title | EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | - |
dc.citation.volume | 114 | - |
dc.citation.startPage | 275 | - |
dc.citation.endPage | 284 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | GROUP BOX 1 | - |
dc.subject.keywordPlus | NF-KAPPA-B | - |
dc.subject.keywordPlus | ANIMAL-MODELS | - |
dc.subject.keywordPlus | SIGNALING PATHWAY | - |
dc.subject.keywordPlus | MICE | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CYTOKINE | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordAuthor | Anti-inflammation | - |
dc.subject.keywordAuthor | Acute lung injury | - |
dc.subject.keywordAuthor | Intratracheal administration | - |
dc.subject.keywordAuthor | Receptor for advanced glycation end-products | - |
dc.subject.keywordAuthor | Nuclear factor-kappa B | - |
dc.subject.keywordAuthor | RAGE-antagonist peptide | - |
dc.identifier.url | https://www.sciencedirect.com/science/article/pii/S0928098717306851?via%3Dihub | - |
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