A curcumin-loaded polymeric micelle as a carrier of a microRNA-21 antisense-oligonucleotide for enhanced anti-tumor effects in a glioblastoma animal model
DC Field | Value | Language |
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dc.contributor.author | Tan, Xiaonan | - |
dc.contributor.author | Kim, Gyeungyun | - |
dc.contributor.author | Lee, Dahee | - |
dc.contributor.author | Oh, Jungju | - |
dc.contributor.author | Kim, Minkyung | - |
dc.contributor.author | Piao, Chunxian | - |
dc.contributor.author | Lee, Jaewon | - |
dc.contributor.author | Lee, Min Sang | - |
dc.contributor.author | Jeong, Ji Hoon | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2021-07-30T05:24:46Z | - |
dc.date.available | 2021-07-30T05:24:46Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2018-02 | - |
dc.identifier.issn | 2047-4830 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4737 | - |
dc.description.abstract | A glioblastoma is a common primary brain tumor that expresses microRNA- 21 (miR-21), which inhibits the expression of pro-apoptotic genes such as phosphatase and tensin homologue (PTEN) and programmed cell death 4 (PDCD4). Therefore, an antisense-oligonucleotide against miR-21 (miR21ASO) could have therapeutic effects for glioblastomas. In this study, curcumin was loaded into deoxycholic acid-conjugated polyethylenimine (DP) micelles. The curcumin-loaded DP micelle (DP-Cur) was evaluated as a carrier for the combined delivery of curcumin and miR21ASO. Gel retardation and heparin competition assays showed that DP-Cur formed stable complexes with miR21ASO. The anti-tumor effects of the combined delivery of curcumin and miR21ASO were evaluated in C6 glioblastoma cells. In vitro transfection showed that DP-Cur had an miR21ASO delivery efficiency similar to that of polyethylenimine (25 kDa, PEI25k) and DP. In the C6 cells, the delivery of miR21ASO using DP-Cur effectively reduced the miR21 level. The miR21ASO/DP-Cur complex induced apoptosis more effectively than the single delivery of curcumin or miR21ASO. The therapeutic effect of the miR21ASO/DP-Cur complex was also evaluated in an intracranial glioblastoma animal model. The miR21ASO/DP-Cur complex reduced the tumor volume more effectively than single therapy of curcumin or miR21ASO. Immunohistochemistry showed that PDCD4 and PTEN were induced in the miR21ASO/DP and miR21ASO/DP-Cur complex groups. Therefore, DP-Cur is an efficient carrier of miR21ASO and the combined delivery of miR21ASO and curcumin may be useful in the development of combination therapy for glioblastoma. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | A curcumin-loaded polymeric micelle as a carrier of a microRNA-21 antisense-oligonucleotide for enhanced anti-tumor effects in a glioblastoma animal model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1039/c7bm01088e | - |
dc.identifier.scopusid | 2-s2.0-85041370203 | - |
dc.identifier.wosid | 000423742400017 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS SCIENCE, v.6, no.2, pp.407 - 417 | - |
dc.relation.isPartOf | BIOMATERIALS SCIENCE | - |
dc.citation.title | BIOMATERIALS SCIENCE | - |
dc.citation.volume | 6 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 407 | - |
dc.citation.endPage | 417 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.subject.keywordPlus | GENE-THERAPY | - |
dc.subject.keywordPlus | HEPATOCELLULAR-CARCINOMA | - |
dc.subject.keywordPlus | ENDOTHELIAL-CELLS | - |
dc.subject.keywordPlus | PEPTIDE MICELLES | - |
dc.subject.keywordPlus | HEME OXYGENASE-1 | - |
dc.subject.keywordPlus | CANCER-THERAPY | - |
dc.subject.keywordPlus | DELIVERY | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | OLIGODEOXYNUCLEOTIDE | - |
dc.identifier.url | https://pubs.rsc.org/en/content/articlelanding/2018/BM/C7BM01088E | - |
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