Anti-cancer effect of R3V6 peptide-mediated delivery of an anti-microRNA-21 antisense-oligodeoxynucleotide in a glioblastoma animal model
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Binna | - |
dc.contributor.author | Song, Hojung | - |
dc.contributor.author | Lee, Dahee | - |
dc.contributor.author | Oh, Jungju | - |
dc.contributor.author | Kim, Gyeungyun | - |
dc.contributor.author | Ihm, Sung-Hee | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2021-07-30T05:25:58Z | - |
dc.date.available | 2021-07-30T05:25:58Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2017-02 | - |
dc.identifier.issn | 1061-186X | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/4815 | - |
dc.description.abstract | MicroRNA-21 (miR-21) expression in glioblastoma inhibits the expression of pro-apoptotic genes, thereby promoting tumor growth. A previous study showed that the amphiphilic R3V6 peptide is an efficient carrier of the anti-miR-21 antisense oligodeoxynucleotide (antisense-ODN) into cells in vitro. In the current study, in vivo delivery of antisense-ODN using the R3V6 peptide was evaluated in a glioblastoma animal model. In vitro transfection showed that the R3V6 peptide delivered antisense-ODN more efficiently than polyethylenimine (25kDa, PEI25k) in C6 glioblastoma cells. For in vivo evaluation, antisense-ODN/R3V6 complex was injected intratumorally into a C6 glioblastoma xenograft animal model. Tumor growth was suppressed by the injection of the antisense-ODN/R3V6 complex, compared with the antisense-ODN/PEI25k and scrambled-antisense-ODN (scr-antisense-ODN)/R3V6 complexes. Real-time RT-PCR showed that miR-21 levels were reduced most efficiently by the antisense-ODNR3V6 complex in tumors. Due to inhibition of miR-21, expression of the programed cell death 4 (PDCD4) gene was promoted in tumors, resulting in the induction of apoptosis of tumor cells. These results suggest that delivery of antisense-ODN using R3V6 peptides may be useful for the development of antisense-ODN therapy for glioblastoma. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.title | Anti-cancer effect of R3V6 peptide-mediated delivery of an anti-microRNA-21 antisense-oligodeoxynucleotide in a glioblastoma animal model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1080/1061186X.2016.1207648 | - |
dc.identifier.scopusid | 2-s2.0-84978513529 | - |
dc.identifier.wosid | 000395193600006 | - |
dc.identifier.bibliographicCitation | JOURNAL OF DRUG TARGETING, v.25, no.2, pp.132 - 139 | - |
dc.relation.isPartOf | JOURNAL OF DRUG TARGETING | - |
dc.citation.title | JOURNAL OF DRUG TARGETING | - |
dc.citation.volume | 25 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 132 | - |
dc.citation.endPage | 139 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | AMPHIPHILIC PEPTIDES | - |
dc.subject.keywordPlus | GENE DELIVERY | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | MICRORNA-21 | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | HYPOXIA | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | SIRNA | - |
dc.subject.keywordPlus | COMPONENT | - |
dc.subject.keywordPlus | INVASION | - |
dc.subject.keywordAuthor | Antisense-oligodeoxynucleotide | - |
dc.subject.keywordAuthor | microRNA-21 | - |
dc.subject.keywordAuthor | R3V6 peptide | - |
dc.subject.keywordAuthor | glioblastoma | - |
dc.subject.keywordAuthor | gene therapy | - |
dc.identifier.url | https://www.tandfonline.com/doi/full/10.1080/1061186X.2016.1207648 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.