Combined Delivery of a Lipopolysaccharide-Binding Peptide and the Heme Oxygenase-1 Gene Using Deoxycholic Acid-Conjugated Polyethylenimine for the Treatment of Acute Lung Injury
DC Field | Value | Language |
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dc.contributor.author | Kim, Ji Yeon | - |
dc.contributor.author | Piao, Chunxian | - |
dc.contributor.author | Kim, Gyeungyun | - |
dc.contributor.author | Lee, Seonyeong | - |
dc.contributor.author | Lee, Min Sang | - |
dc.contributor.author | Jeong, Ji Hoon | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2021-07-30T05:33:14Z | - |
dc.date.available | 2021-07-30T05:33:14Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2017-08 | - |
dc.identifier.issn | 1616-5187 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/5387 | - |
dc.description.abstract | A ternary complex comprising plasmid DNA, lipopolysaccharide-binding peptide (LBP), and deoxycholic acid-conjugated polyethylenimine (PEI-DA) is prepared for combinational therapy of acute lung injury (ALI). The LBP is designed as an anti-inflammatory peptide based on the lipopolysaccharide (LPS)-binding domain of HMGB-1. In vitro cytokine assays show that LBP reduces levels of proinflammatory cytokines by inhibiting LPS. PEI-DA is synthesized as the gene carrier by conjugation of deoxycholic acid to low-molecular weight polyethy-lenimine (2 kDa, PEI2k). PEI-DA has higher transfection efficiency than high-molecular weight polyethylenimine (25 kDa, PEI25k). The ternary complex of an HO-1 plasmid (pHO-1), PEI-DA, and LBP is prepared as a combinational system to deliver the therapeutic gene and peptide. The transfection efficiency of the ternary complex is higher than that of the pHO-1/PEI-DA binary complex. The ternary complex also reduces TNF-alpha secretion in LPS-activated Raw264.7 macrophage cells. Administration of the ternary complex into the lungs of an animal ALI model by intratracheal injection induces HO-1 expression and reduces levels of proinflammatory cytokines more efficiently than the pHO-1/PEI-DA binary complex or LBP alone. In addition, the ternary complex reduces inflammation in the lungs. Therefore, the pHO-1/PEI-DA/LBP ternary complex may be an effective treatment for ALI. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY-V C H VERLAG GMBH | - |
dc.title | Combined Delivery of a Lipopolysaccharide-Binding Peptide and the Heme Oxygenase-1 Gene Using Deoxycholic Acid-Conjugated Polyethylenimine for the Treatment of Acute Lung Injury | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1002/mabi.201600490 | - |
dc.identifier.scopusid | 2-s2.0-85019479108 | - |
dc.identifier.wosid | 000409001400007 | - |
dc.identifier.bibliographicCitation | MACROMOLECULAR BIOSCIENCE, v.17, no.8 | - |
dc.relation.isPartOf | MACROMOLECULAR BIOSCIENCE | - |
dc.citation.title | MACROMOLECULAR BIOSCIENCE | - |
dc.citation.volume | 17 | - |
dc.citation.number | 8 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalResearchArea | Polymer Science | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.relation.journalWebOfScienceCategory | Polymer Science | - |
dc.subject.keywordPlus | WATER-SOLUBLE LIPOPOLYMER | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | IN-VIVO | - |
dc.subject.keywordPlus | HMGB1 | - |
dc.subject.keywordPlus | POLY(ETHYLENIMINE) | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | CARRIER | - |
dc.subject.keywordPlus | STRESS | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordAuthor | acute lung injury | - |
dc.subject.keywordAuthor | anti-inflammation | - |
dc.subject.keywordAuthor | gene delivery | - |
dc.subject.keywordAuthor | heme oxygenase-1 | - |
dc.subject.keywordAuthor | lipopolysaccharide-binding peptide | - |
dc.identifier.url | https://onlinelibrary.wiley.com/doi/10.1002/mabi.201600490 | - |
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