Delivery of Hypoxia-Inducible Heme Oxygenase-1 Gene for Site-Specific Gene Therapy in the Ischemic Stroke Animal Model
DC Field | Value | Language |
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dc.contributor.author | Choi, Manbok | - |
dc.contributor.author | Oh, Jungju | - |
dc.contributor.author | Rhim, Taiyoun | - |
dc.contributor.author | Lee, Minhyung | - |
dc.date.accessioned | 2021-07-30T05:34:06Z | - |
dc.date.available | 2021-07-30T05:34:06Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2016-09 | - |
dc.identifier.issn | 0724-8741 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/5510 | - |
dc.description.abstract | To reduce side effects due to non-specific expression, the heme oxygenase-1 (HO-1) gene under control of a hypoxia-inducible erythropoietin (Epo) enhancer (pEpo-SV-HO-1) was developed for site-specific gene therapy of ischemic stroke. pEpo-SV-HO-1 was constructed by insertion of the Epo enhancer into pSV-HO-1. Dexamethasone-conjugated polyamidoamine (PAMAM-Dexa) was used as a gene carrier. In vitro transfection assays were performed in the Neuro2A cells. In vivo efficacy of pEpo-SV-HO-1 was evaluated in the transient middle cerebral artery occlusion (MCAO) model. In vitro transfection assay with the PAMAM-Dexa/pEpo-SV-HO-1 complex showed that pEpo-SV-HO-1 had higher HO-1 gene expression than pSV-HO-1 under hypoxia. In addition, pEpo-SV-HO-1 reduced the level of apoptosis more efficiently than pSV-HO-1 in Neuro2A cells under hypoxia. For in vivo evaluation, the PAMAM-Dexa/pEpo-SV-HO-1 complex was injected into the ischemic brain of the transient MCAO model. pEpo-SV-HO-1 increased HO-1 expression and reduced the number of apoptotic cells in the ischemic brain, compared with the pSV-HO-1 injection group. As a result, the infarct volume was more efficiently decreased by pEpo-SV-HO-1 than by pSV-HO-1. pEpo-SV-HO-1 induced HO-1 gene expression and therapeutic effect in the ischemic brain. Therefore, pEpo-SV-HO-1 may be useful for site-specific gene therapy of ischemic stroke. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | SPRINGER/PLENUM PUBLISHERS | - |
dc.title | Delivery of Hypoxia-Inducible Heme Oxygenase-1 Gene for Site-Specific Gene Therapy in the Ischemic Stroke Animal Model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Rhim, Taiyoun | - |
dc.contributor.affiliatedAuthor | Lee, Minhyung | - |
dc.identifier.doi | 10.1007/s11095-016-1962-9 | - |
dc.identifier.scopusid | 2-s2.0-84975302305 | - |
dc.identifier.wosid | 000381260000016 | - |
dc.identifier.bibliographicCitation | PHARMACEUTICAL RESEARCH, v.33, no.9, pp.2250 - 2258 | - |
dc.relation.isPartOf | PHARMACEUTICAL RESEARCH | - |
dc.citation.title | PHARMACEUTICAL RESEARCH | - |
dc.citation.volume | 33 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 2250 | - |
dc.citation.endPage | 2258 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.subject.keywordPlus | DEXAMETHASONE-CONJUGATED POLYETHYLENIMINE | - |
dc.subject.keywordPlus | SPINAL-CORD-INJURY | - |
dc.subject.keywordPlus | BRAIN | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | SYSTEM | - |
dc.subject.keywordPlus | CARDIOMYOCYTES | - |
dc.subject.keywordPlus | PROTECTION | - |
dc.subject.keywordPlus | CARRIER | - |
dc.subject.keywordPlus | LIPOPOLYMER | - |
dc.subject.keywordPlus | DENDRIMERS | - |
dc.subject.keywordAuthor | gene delivery | - |
dc.subject.keywordAuthor | heme oxygenase-1 | - |
dc.subject.keywordAuthor | hypoxia-inducible gene | - |
dc.subject.keywordAuthor | ischemic stroke | - |
dc.subject.keywordAuthor | site-specific gene therapy | - |
dc.identifier.url | https://link.springer.com/article/10.1007/s11095-016-1962-9 | - |
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