Bioinspired tuning of glycol chitosan for 3D cell culture
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, Myeong Ok | - |
dc.contributor.author | Li, Zhengzheng | - |
dc.contributor.author | Shim, Hye-Eun | - |
dc.contributor.author | Cho, Ik-Sung | - |
dc.contributor.author | Nurunnabi, Md | - |
dc.contributor.author | Park, Honghyun | - |
dc.contributor.author | Lee, Kuen Yong | - |
dc.contributor.author | Moon, Sung-Hwan | - |
dc.contributor.author | Kim, Ki-Suk | - |
dc.contributor.author | Kang, Sun-Woong | - |
dc.contributor.author | Huh, Kang Moo | - |
dc.date.accessioned | 2021-07-30T05:34:07Z | - |
dc.date.available | 2021-07-30T05:34:07Z | - |
dc.date.created | 2021-05-12 | - |
dc.date.issued | 2016-09 | - |
dc.identifier.issn | 1884-4049 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/5511 | - |
dc.description.abstract | Three-dimensional (3D) cell culture systems have promising applications compared with conventional two-dimensional cell culture systems. Herein, we report a facile method for the formation of 3D spheroids using novel thermo-reversible polysaccharide-based hydrogels. A series of thermo-reversible hydrogels consisting of N-acyl glycol chitosans (NAGCs) are synthesized through a simple N-acylation reaction, and the degree of acylation is finely tuned to obtain adequate thermo-reversible properties and gel stability. Among the NAGCs, N-hexanoyl glycol chitosan is the most thermo-sensitive and is highly effective for forming multi-cellular spheroids when used to coat the surfaces of cell culture dishes. Cell spheroids are effectively formed at various cell concentrations, and their spheroid shape and cellular functions are well maintained for longer times. The hydrogel culture system is also useful for co-cultures that mimic a biological microenvironment. Our thermo-reversible hydrogels may offer a convenient method for the development of in vitro 3D cell culture systems to provide enhanced performance in tissue regeneration, organ-on-chips, drug screening research and other biomedical applications. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | Bioinspired tuning of glycol chitosan for 3D cell culture | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Lee, Kuen Yong | - |
dc.identifier.doi | 10.1038/am.2016.130 | - |
dc.identifier.scopusid | 2-s2.0-85041955885 | - |
dc.identifier.wosid | 000383415300008 | - |
dc.identifier.bibliographicCitation | NPG ASIA MATERIALS, v.8, pp.1 - 10 | - |
dc.relation.isPartOf | NPG ASIA MATERIALS | - |
dc.citation.title | NPG ASIA MATERIALS | - |
dc.citation.volume | 8 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 10 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.subject.keywordPlus | HAIR FOLLICLE REGENERATION | - |
dc.subject.keywordPlus | SPHEROIDS | - |
dc.subject.keywordPlus | MEMBRANES | - |
dc.subject.keywordPlus | COCULTURE | - |
dc.subject.keywordPlus | MATRICES | - |
dc.subject.keywordPlus | SYSTEMS | - |
dc.identifier.url | https://www.nature.com/articles/am2016130 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.