Dexamethasone conjugated polyethylenimine as a carrier of plasmid encoding heme oxygenase-1 for stroke gene therapy
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2021-08-03T21:34:40Z | - |
dc.date.available | 2021-08-03T21:34:40Z | - |
dc.date.created | 2021-06-30 | - |
dc.date.issued | 2009-07-05 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/61324 | - |
dc.description.abstract | Dexamethasone is one of the anti-inflammatory glucocorticoid steroids. Previously, we synthesized dexamethasone conjugated polyethylenimine (PEI-Dexa) as a gene carrier with anti-inflammatory effect. In this study, HO-1 gene was delivered to focal ischemic brain of stroke animal model using PEI-Dexa as a gene carrier. Heme oxygenase-1 (HO-1) is an anti-apoptotic protein, which can protect cell from apoptosis under hypoxia. In divided groups of rats (n=9), pCI/PEI, pCI/PEI-Dexa and pEpo-SV-HO-1/PEI-Dexa complexes were injected directly into the cortex 1 hr prior to middle cerebral artery occlusion (MCAO) except control group. MCAO was performed for 60 min in male Sprague Dawley (SD) rats (280-320g). Also, cortical blood flow was continuously monitored by Laser Doppler Flowmetry. At 24 hrs after MCAO, infarct size was evaluated by 2% 2,3,5-triphenyltetrazolium hydrochloride (TTC) staining. The level of cytokines such as TNF-α, IL-1β were measured by ELISA in the infarcted area. ELISA for the HO-1 protein was also performed. The results showed that the injection of pCI/PEI-Dexa complex reduced infarct size significantly, compared with the control and pCI/PEI injection groups. This suggests that dexamethsone segment of PEI-Dexa protects the brain cells under ischemia-reperfusion condition. Also, HO-1 expression was induced in the pEpo-SV-HO-1/PEI-Dexa injection group. The infarct size of the pEpo-SV-HO-1/PEI-Dexa injection group was further reduced compared with the pCI/PEI-Dexa injection group, suggesting that HO-1 has synergistic effect with PEI-Dexa in reducing inflammatory response in ischemic brain. Therefore, pEpo-SV-HO-1/PEI-Dexa complex is a potential candidate for therapeutic gene delivery to ischemic brain. | - |
dc.publisher | Federation of European Biochemical Societies | - |
dc.title | Dexamethasone conjugated polyethylenimine as a carrier of plasmid encoding heme oxygenase-1 for stroke gene therapy | - |
dc.type | Conference | - |
dc.contributor.affiliatedAuthor | 이민형 | - |
dc.identifier.bibliographicCitation | 34th FEBS Congress | - |
dc.relation.isPartOf | 34th FEBS Congress | - |
dc.citation.title | 34th FEBS Congress | - |
dc.citation.conferencePlace | 체코 프라하 | - |
dc.type.rims | CONF | - |
dc.description.journalClass | 1 | - |
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