The feasibility of β-TCP as an osteogenic carrier for osteoblast-like cells induced from bone marrow-derived mesenchymal stem cells in vitro

Abstract

The present study aimed to investigate the feasibility of β-TCP as an osteogenic carrier for osteoblast-like cells (OCs) induced from bone marrow-derived mesenchymal stem cells (MSCs) in vitro. Osteoblast differentiation was induced in confluent cultures of MSCs by adding 100 nM dexamethasone, 10 mM β-glycerophosphate, and 50 mM L-ascorbic acid. The Alizarin red S staining and reverse transcriptase-polymerase chain reaction (RT-PCR) were performed to examine the mRNA expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), receptor activator for nuclear factor κB ligand (RANKL), runt-related transcription factor 2 (RUNX2), collgen-I (COL-I). There were no significant differences in the osteogenic potentials of OCs induced from MSCs on β-TCP(+/-). According to the incubation period, there were significant increasing of Alizarin red S staining in the incubation 3 weeks. The mRAN expression of ALP, RUNX2, and RANKL were higher in OCs/β-TCP(-) than OCs/β-TCP(+). Our study presented the β-TCP will have the possibility that could directly affect the osteoblastic differentiation of the bone marrow-derived MSCs.