The feasibility of β-TCP as an osteogenic carrier for osteoblast-like cells induced from bone marrow-derived mesenchymal stem cells in vitro
DC Field | Value | Language |
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dc.contributor.author | 한지영 | - |
dc.date.accessioned | 2021-08-03T22:20:07Z | - |
dc.date.available | 2021-08-03T22:20:07Z | - |
dc.date.created | 2021-06-30 | - |
dc.date.issued | 2009-02-26 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/62354 | - |
dc.description.abstract | The present study aimed to investigate the feasibility of β-TCP as an osteogenic carrier for osteoblast-like cells (OCs) induced from bone marrow-derived mesenchymal stem cells (MSCs) in vitro. Osteoblast differentiation was induced in confluent cultures of MSCs by adding 100 nM dexamethasone, 10 mM β-glycerophosphate, and 50 mM L-ascorbic acid. The Alizarin red S staining and reverse transcriptase-polymerase chain reaction (RT-PCR) were performed to examine the mRNA expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), receptor activator for nuclear factor κB ligand (RANKL), runt-related transcription factor 2 (RUNX2), collgen-I (COL-I). There were no significant differences in the osteogenic potentials of OCs induced from MSCs on β-TCP(+/-). According to the incubation period, there were significant increasing of Alizarin red S staining in the incubation 3 weeks. The mRAN expression of ALP, RUNX2, and RANKL were higher in OCs/β-TCP(-) than OCs/β-TCP(+). | - |
dc.publisher | the Academy of Osseointegration | - |
dc.title | The feasibility of β-TCP as an osteogenic carrier for osteoblast-like cells induced from bone marrow-derived mesenchymal stem cells in vitro | - |
dc.type | Conference | - |
dc.contributor.affiliatedAuthor | 한지영 | - |
dc.identifier.bibliographicCitation | The 24th Annual Meeting of the Academy of Osseointegration | - |
dc.relation.isPartOf | The 24th Annual Meeting of the Academy of Osseointegration | - |
dc.citation.title | The 24th Annual Meeting of the Academy of Osseointegration | - |
dc.citation.conferencePlace | San Diego | - |
dc.type.rims | CONF | - |
dc.description.journalClass | 1 | - |
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