Targetede delivery of siRNA in vivo
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이상경 | - |
dc.date.accessioned | 2021-08-03T22:32:59Z | - |
dc.date.available | 2021-08-03T22:32:59Z | - |
dc.date.created | 2021-06-30 | - |
dc.date.issued | 2008-11-28 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/62679 | - |
dc.description.abstract | Background: RNA interference (RNAi) is increasingly being considered as a promising approach in the therapy of a variety of human diseases including cancers, viral infections, degenerative diseases, and inflammation. However, for RNAi to become a feasible clinical application, a major obstacle is efficient delivery of small interfering RNAs (siRNA) to specific target cells and tissues. Targeted delivery of siRNA is essential for maximizing the efficiency of gene-silencing while reducing unwanted side effects. We demonstrate two methods for the delivery of siRNA to two different target cell types in animal models, via neurons and T cells, primary cells that are extremely resistant to transfection with nucleic acids. Methods: Rabies virus glycoprotein (RVG) derived peptide was used as a ligand for neuronal cell specific delivery mediated by its binding to the alpha 7-subunit of nicotinic acetylcholine receptor. Along the same lines, a single chain antibody (scFvCD7) directed to the pan T cell molecule, CD7 was used for specific siRNA delivery to human T cells. The siRNA binding was enabled by poly 9-Arginine conjugated to the targeting moiety. Results and Discussions: RVG-9R specifically delivered siRNA into neuronal cells and prevented CNS infection with Japanese encephalitis virus in mice. scFvCD7-9R delivered siRNA specifically to human T cells and controlled HIV infection in humanized mice. Thus we have successfully targeted 2 important target cell types for the cell-specific delivery of siRNA and possibly DNA and drugs as well. Part of this work supported by Korea Ministry of Education and Science Technology (MEST) | - |
dc.publisher | 한국생체재료학회 | - |
dc.title | Targetede delivery of siRNA in vivo | - |
dc.type | Conference | - |
dc.contributor.affiliatedAuthor | 이상경 | - |
dc.identifier.bibliographicCitation | 2008년 한국생체재료학회 제 16차 학술대회 | - |
dc.relation.isPartOf | 2008년 한국생체재료학회 제 16차 학술대회 | - |
dc.citation.title | 2008년 한국생체재료학회 제 16차 학술대회 | - |
dc.citation.conferencePlace | 서울 테크노파크 6층 | - |
dc.type.rims | CONF | - |
dc.description.journalClass | 2 | - |
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