Targeted delivery of siRNA to human T cells in humanized mice
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이상경 | - |
dc.date.accessioned | 2021-08-04T00:19:46Z | - |
dc.date.available | 2021-08-04T00:19:46Z | - |
dc.date.created | 2021-06-30 | - |
dc.date.issued | 2008-03-27 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/hanyang/handle/2021.sw.hanyang/65314 | - |
dc.description.abstract | siRNA delivery to T lymphocytes remains a major hurdle for harnessing RNAi as a therapeutic strategy, especially in HIV infections. A single chain antibody specific to a pan T cell surface antigen CD7 (scFvCD7) conjugated to a positively charged oligo-9-arginine peptide was used for targeted delivery of siRNA to T cells. The approach was tested in NOD/SCIDIL2r c-/- mice reconstituted with human peripheral blood lymphocytes (Hu-PBL) or hematopoietic stem cells (Hu-HSC). Intravenous administration of scFvCD7-9R/CD4siRNA complexes resulted in efficient CD4 gene silencing in human T cells. In HIV-infected Hu-PBL mice, treatment with a combination of siRNAs targeting cellular CCR5 and viral genes complexed to scFvCD7-9R efficiently controlled viral replication and prevented CD4 T cell loss for up to 5 weeks after infection. Strikingly, this approach also suppressed endogenous virus in mice reconstituted with HIV seropositive PBMC leading to restoration of CD4 T cell counts. Moreover, scFvCD7-9R could also deliver siRNAs to naive T cells in Hu-HSC mice, rendering them resistant to HIV-challenge ex vivo. Our data suggest that scFvCD7-9R is an efficient tool for the delivery of siRNA to T cells in addition to being a promising approach for RNAi-mediated therapy of HIV-1. | - |
dc.publisher | Keystone Symposia | - |
dc.title | Targeted delivery of siRNA to human T cells in humanized mice | - |
dc.type | Conference | - |
dc.contributor.affiliatedAuthor | 이상경 | - |
dc.identifier.bibliographicCitation | RNAi, microRNA and Non-coding RNA | - |
dc.relation.isPartOf | RNAi, microRNA and Non-coding RNA | - |
dc.citation.title | RNAi, microRNA and Non-coding RNA | - |
dc.citation.conferencePlace | Canada | - |
dc.type.rims | CONF | - |
dc.description.journalClass | 1 | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
222, Wangsimni-ro, Seongdong-gu, Seoul, 04763, Korea+82-2-2220-1365
COPYRIGHT © 2021 HANYANG UNIVERSITY.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.